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EC number: 216-721-0 | CAS number: 1653-19-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- circa 1968
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Publication has limited information on methods and the study was conducted prior to the introduction of GLP guidelines or standardised test methods.
Cross-reference
- Reason / purpose for cross-reference:
- reference to same study
Data source
Reference
- Reference Type:
- publication
- Title:
- Toxic action of dichlorobutadiene in single experiments. Mater Of itogovoi Of nauch Of konf Of vop Of gig Tr Of profpatol Of khim Of gornorud Of prom
- Author:
- Grigoryan, A. O.
- Year:
- 1 968
- Bibliographic source:
- Chem. Abstr. 72: 119600x (1970) and in the review of DuPont, updated by R.C. Graham, January 21, 1982.
Materials and methods
Test guideline
- Qualifier:
- no guideline available
- Principles of method if other than guideline:
- The study design was similar to the subsequently defined OED method 401. The standard requirements for determination of a median lethal oral dose level were included - groups of male and female rats or mice, acutely dosed at various dose levels and mortality patterns evaluated.
- GLP compliance:
- no
- Test type:
- other: design similar to OECD method 401 - median lethal dose determination
- Limit test:
- no
Test material
- Reference substance name:
- 2,3-dichlorobuta-1,3-diene
- EC Number:
- 216-721-0
- EC Name:
- 2,3-dichlorobuta-1,3-diene
- Cas Number:
- 1653-19-6
- Molecular formula:
- C4H4Cl2
- IUPAC Name:
- 2,3-dichlorobuta-1,3-diene
Constituent 1
Test animals
- Species:
- rat
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source:
- Age at study initiation:
- Weight at study initiation:
- Fasting period before study:
- Housing:
- Diet (e.g. ad libitum):
- Water (e.g. ad libitum):
- Acclimation period:
ENVIRONMENTAL CONDITIONS
- Temperature (°C):
- Humidity (%):
- Air changes (per hr):
- Photoperiod (hrs dark / hrs light):
IN-LIFE DATES: From: To:
Administration / exposure
- Route of administration:
- oral: gavage
- Details on oral exposure:
- VEHICLE
- Concentration in vehicle:
- Amount of vehicle (if gavage):
- Justification for choice of vehicle:
- Lot/batch no. (if required):
- Purity:
MAXIMUM DOSE VOLUME APPLIED:
DOSAGE PREPARATION (if unusual):
- Doses:
-
100, 500 and 600 mg/kg bw - Details on study design:
- - Duration of observation period following administration: 14 days (or other?)
- Frequency of observations and weighing:
- Necropsy of survivors performed: yes/no
- Other examinations performed: clinical signs, body weight,organ weights, histopathology, other:
Results and discussion
- Preliminary study:
- Not applicable
Effect levelsopen allclose all
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- ca. 222 mg/kg bw
- Remarks on result:
- other: rats
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- ca. 110 mg/kg bw
- Remarks on result:
- other: mice
- Mortality:
- Deaths occurred at dose levels of 100 mg/kg bw and above. Deaths occurred within 2 days of dosing (80% of unscheduled deaths) or within 4 days of dosing.
- Gross pathology:
- Examination of animals which died during the first 2 days after application revealed polyhemia of all internal organs. The liver was nutmeg-like enlarged with a dark coloured central part and pale edges. In most cases the spleen was a dark brown colour and enlarged with dull edges. The stomach was full, dilated, and had the characteristic odour of 2,3-dichlorobuta-1,3-diene.
- Other findings:
- In the animals sacrificed at the end of the observation period some enlargement of the spleen was observed macroscopically while the other internal organs appeared normal. Microscopic examination of the internal organs revealed deep or superficial necrotic changes of the mucous envelope of the stomach with polyhemia as well as protein and fatty dystrophy of the epithelial tissues of the kidneys of the animals of the 500 and 600 mg/kg bw groups.
Applicant's summary and conclusion
- Interpretation of results:
- harmful
- Remarks:
- Migrated information Criteria used for interpretation of results: EU
- Conclusions:
- The LD50 values for rats and mice were determined as 222 mg/kg bw and 110 mg/kg bw respectively. This indicates DCBD should be considered to be harmful. Toxic effects were observed as clinical signs of reaction to treatment, including mortality, pathological changes in the liver, spleen and stomach with associated histopathological changes.
- Executive summary:
In rats and mice the LD50 was determined as 222 and 110 mg/kg bw, respectively. Immediately after application of 2,3-dichlorobuta-1,3-diene of unknown purity the animals became slightly more active followed quickly by depression, low mobility and indifference to food and environmental stimuli. At 100 mg/kg bw and above deaths occurred (80 % of the deaths during the first 2 days and 20 % during the next 3-4 days); 600 mg/kg bw were absolutely lethal. Examination of animals which died during the first 2 days after application revealed polyhemia of all internal organs. The liver was nutmeg-like enlarged with a dark coloured central part and pale edges. In most cases the spleen was dark brown coloured and enlarged with dull edges. The stomach was full, dilated, and had the characteristic odour of 2,3-dichlorobuta-1,3-diene. No significant pathological changes were observed in the other organs. In the animals sacrificed at the end of the observation period some enlargement of the spleen was observed macroscopically while the other internal organs appeared normal. Microscopic examination of the internal organs revealed deep or superficial necrotic changes of the mucous envelope of the stomach with polyhemia as well as protein and fatty dystrophy of the epithelial tissues of the kidneys of the animals of the 500 and 600 mg/kg bw groups.
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