3-aminomethyl-3,5,5-trimethylcyclohexylamine

Biodegradation in water and sediment: simulation tests

Administrative data

Endpoint:

biodegradation in water: sewage treatment simulation testing

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1992-07-07 to 1992-08-07

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Guideline study with acceptable restrictions: Distinction between elimination by biodegradation and by adsorption not possible

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1992

Report date:

1992

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 303 A (Simulation Test - Aerobic Sewage Treatment. A: Activated Sludge Units)

Deviations:

not specified

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

3-aminomethyl-3,5,5-trimethylcyclohexylamine

EC Number:

220-666-8

EC Name:

3-aminomethyl-3,5,5-trimethylcyclohexylamine

Cas Number:

2855-13-2

Molecular formula:

C10H22N2

IUPAC Name:

3-(aminomethyl)-3,5,5-trimethylcyclohexan-1-amine

Radiolabelling:

no

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, non-adapted

Details on inoculum:

INOCULUM/TEST ORGANISM
- Source: municipal WWTP Marl-West, sampled 07 Jul 1992
- Pretreatment: fed into test apparatus ca. 40 min after sampling

Duration of test (contact time):

6 d

Initial test substance concentration

Initial test substance concentration 1

Initial conc.:

15.9 mg/L

Based on:

test mat.

Initial test substance concentration 2

Initial conc.:

10.1 mg/L

Based on:

DOC

Parameter followed for biodegradation estimation:

DOC removal

Details on study design:

TEST SYSTEM
- Culturing apparatus: flow-through (3 l; 0.5 l/h) - Number of culture flasks per concentration: 1 - Aeration device: pump

- Composition of synthetic waste water: 88 mg/l Pepton (Unipath) 55 mg/l meat extract (Unipath) 15 mg/l urea, CAS RN 57-13-6 3.5 mg/l sodium chloride p.a., CAS RN 7647-14-5 2 mg/l calcium chloride x 2 H2O p.a. 1 mg/l magnesium sulfate x 7 H2O p.a. 14 mg/l K2HPO4 p.a. 98 mg/l NaHCO3 (Ferak) -
Additional nutrition substrate A: 32 g/l Pepton 22 g/l meat extract 6 g/l urea 1.4 g/l sodium chloride 0.8 g/l calcium chloride x 2 H2O 0.4 g/l magnesium sulfate x 7 H2O substrate B
- 33.5 g/l K2HPO4 is stored separately - 47 g/l NaHCO3 is stored separately
- 5 ml K2HPO4 soln. + 25 ml NaHCO3 soln + 11 l tap water This soln. and nutrition substrate A (30 ml/l) are added separately

- Duration of the test:: 31 days

- Test temperature: 21.8-26.2 degree C - Other relevant factors: mean retention time 6 hours

Reference substance

Reference substance:

not required

Results and discussion

% Degradation

% Degr.:

42

Parameter:

DOC removal

Sampling time:

31 d

Transformation products:

not measured

Evaporation of parent compound:

not measured

Volatile metabolites:

not measured

Residues:

not measured

Details on results:

The mean of 19 measurements at approximately regular intervals is 42.0 +/- 5.01 % degradation

Applicant's summary and conclusion

Validity criteria

Validity criteria fulfilled:

not specified

Conclusions:

The present study indicates that a certain amount (ca. 42 %) of the test substance may degrade in the sewage treatment plant.

Executive summary:

The present study on isophorone diamine simulates the degradation of the test substance in a sewage treatment plant and is similar or equivalent to OECD 303. The simulation study indicates that 42 % of the test item degraded within a period of 31 days. The study was assessed as "reliable with restrictions".