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Diss Factsheets
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EC number: 244-073-9 | CAS number: 20837-86-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Eye irritation
Administrative data
- Endpoint:
- eye irritation: in vitro / ex vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: study was preformed according to GLP
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 010
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: ICCVAm Test Method Evaluation Report: Appendix G ICCVAM recommended HET-CAM Method Protocol (Nov. 2006)
- Principles of method if other than guideline:
- This "in vitro study" is preformed to assess the irritating potential of Blei-cyanamid by detection of damages in blood vessels under the chorioallantoic membrane of nine day incubated chicken eggs.
- GLP compliance:
- yes
Test material
- Reference substance name:
- Lead cyanamidate
- EC Number:
- 244-073-9
- EC Name:
- Lead cyanamidate
- Cas Number:
- 20837-86-9
- Molecular formula:
- CN2.Pb
- IUPAC Name:
- λ²-lead(2+) (azanidylenemethylidene)azanide
Constituent 1
Test animals / tissue source
- Species:
- other: Lohman Leghorn chicken eggs
- Strain:
- not specified
- Details on test animals or tissues and environmental conditions:
- Freshly laid Lohmann Leghorn chicken eggs will be obtained from the LSL Rhein Main Geflügelvermehrungsbetrieb (Dieburg, Germany) nine days before the start of the test (the day of delivery will be stated in the final report).
Incubation
The eggs are incubated at 38.3 ± 0.2 °C and 58 ± 2% humidity for eight days in a still air incubator. During incubation, the eggs are rotated to prevent an attachment of the embryo to one side of the egg.
Test system
- Vehicle:
- water
- Details on study design:
- Preparation of Test System
24 hours before the start of the experiment (eight days after obtaining the eggs), the chicken eggs are candled and non-viable eggs are discarded. The rest of the eggs is placed upward in the incubator until the next day.
On the day of the test, the eggs are removed from the incubator for use in the assay.
At first, the eggs are weighed and only eggs with a weight between 50g and 60g are used. All other eggs are discarded. Then, the eggs are candled and the air bubble is marked. The egg is opened on the air bubble with a rotating dentist’s saw blade or tweezers. The inner membrane is moistened with 0.9 % NaCl; then, the egg is left to stand into the incubator for 30 minutes. After this period, the solution is decanted and the inner membrane is carefully removed with a forceps.
Description of the Method
The test item resp. the negative control resp. the positive control is given directly onto the CAM surface.
For each replicate of the liquid negative and positive controls, 300 µL are measured using an adjustable pipette with single-use tips.
For each test item replicate, 300 µL are measured using a centrifuge vial and weighed. If the weight surpasses 300 mg, six replicates with 300 ± 30 mg each are weighed for the test. If the weight is less than 300 mg, six replicates with 300 µL each are weighed for the test.
Number of eggs which are used for the test item: 6
Number of eggs which are used for the negative control: 3
Number of eggs which are used for each positive control: 3
The reactions on the CAM are observed over a period of 301 seconds.
The following endpoints are described in the guideline:
• haemorrhage (bleeding from the vessels)
• vascular lysis (blood vessel disintegration)
• coagulation (intra- and extra-vascular protein denaturation)
The time for the appearance of each of the noted endpoints is monitored and recorded in seconds.
Results and discussion
Any other information on results incl. tables
The substance lead cyanamid can be regarded as severely irritant.
Applicant's summary and conclusion
- Interpretation of results:
- other: severly irritant
- Conclusions:
- The presented data are reliable and adequate. The EU-Method is an international accepted method. This method had been chosen to avoid unnecessary animal experiments.
- Executive summary:
The substance lead cyanamid can be regarded as severely irritant.
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