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Toxicological information

Acute Toxicity: oral

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Administrative data

Endpoint:
acute toxicity: oral
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Non-guideline, non-GLP study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1975
Report date:
1975

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 401 (Acute Oral Toxicity)
GLP compliance:
no
Test type:
standard acute method
Limit test:
no

Test material

Constituent 1
Reference substance name:
Phosphorodithioic acid, O,O-bis(2-ethylhexyl) ester, zinc salt
IUPAC Name:
Phosphorodithioic acid, O,O-bis(2-ethylhexyl) ester, zinc salt
Constituent 2
Chemical structure
Reference substance name:
Zinc bis[O,O-bis(2-ethylhexyl)] bis(dithiophosphate)
EC Number:
224-235-5
EC Name:
Zinc bis[O,O-bis(2-ethylhexyl)] bis(dithiophosphate)
Cas Number:
4259-15-8
Molecular formula:
Too complex
IUPAC Name:
1-Hexanol, 2-ethyl-, O,O-diester with phosphorodithioic acid, zinc salt

Test animals

Species:
rat
Strain:
Sprague-Dawley
Sex:
male
Details on test animals or test system and environmental conditions:
TEST ANIMALS
- Source:
- Age at study initiation:
- Weight at study initiation: 232-296 g
- Fasting period before study:
- Housing: Housed individually in wire bottom cages
- Diet (e.g. ad libitum): ad libitum except overnight prior to dosing
- Water (e.g. ad libitum): ad libitum except overnight prior to dosing
- Acclimation period:


ENVIRONMENTAL CONDITIONS
- Temperature (°C): 21.1
- Humidity (%):
- Air changes (per hr):
- Photoperiod (hrs dark / hrs light):


IN-LIFE DATES: From: To:

Administration / exposure

Route of administration:
oral: gavage
Vehicle:
unchanged (no vehicle)
Doses:
2200 mg/kg, 3300 mg/kg, 5000 mg/kg, 7500 mg/kg
No. of animals per sex per dose:
10 male
Control animals:
yes
Details on study design:
- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: 7 and 14 days
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight, gross pathology including: thymus, heart, lungs, liver, kidneys, adrenal glands, spleen, gonads, gastro-intestinal tract, lymph nodes, pancreas, salivary glands, bladder, body fat, skeletal muscle, teeth, eyes and skin.
Statistics:
Berkson, J. Biometrika, 44:411-435, 1957

Results and discussion

Effect levels
Sex:
male
Dose descriptor:
LD50
Effect level:
3 100 mg/kg bw
95% CL:
1 800 - 5 100
Mortality:
7500 mg/kg: 10/10 rats died 1-2 days after exposure
5000 mg/kg: 9/10 rats died between 1-9 days after exposure
3300 mg/kg: 6/10 rats died 1-2 days after exposure
2200 mg/kg: 2/10 rats died between 1-8 days after exposure
Clinical signs:
Depresson, diarrhea, reduced food intake
Gross pathology:
Survivors had less than normal amounts of body fat. No other chnages observed.

Applicant's summary and conclusion

Interpretation of results:
Category 5 based on GHS criteria
Remarks:
Migrated information