Formaldehyde, oligomeric reaction products with aniline

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: GLP guideline study without analytical monitoring

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1992

Report date:

1992

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: National industrial standard test guideline DIN 38 412 part 9

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Phenylbase MDA 70

IUPAC Name:

Phenylbase MDA 70

Details on test material:

- Name of test material (as cited in study report): Phenylbase MDA 70
- Molecular formula (if other than submission substance):
- Composition of test material, percentage of components: 4,4'-Diaminodiphenylmethan (59.7%), 2,4'-Diaminodiphenylmethan (2.7%), 2,2'-Diaminodiphenylmethan (0.06%), 3-core-MDA-Isomers (21.3%), 4-core-MDA-isomers (7.9%), 5-core-MDA-isomers (3.3%), multiple core MDA-isomers (3.9%)
- Lot/batch No.: A 90/0073/00 UER

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: test substance directly applied to test medium

Test organisms

Test organisms (species):

Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)

Details on test organisms:

TEST ORGANISM
- Common name: Green algae
- Source (laboratory, culture collection): Pflanzenphysiologisches Institut der Universität, 3400 Göttingen
- Method of cultivation: In a light chamber at 23 ± 2 °C at an illumination of 120 µEinstein/m2*s

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

72 h

Test conditions

pH:

8.3 - 10.7

Nominal and measured concentrations:

nominal: control, 3.2, 10, 32, 100, 320 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: 300 mL Erlenmeyer flasks
- Material, size, headspace, fill volume: glass, fill volume 100 mL
- Initial cells density: 10000 cells/mL
- Control end cells density:404000 cells/mL

GROWTH MEDIUM
- Standard medium used: yes

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: Microcellcounter Sysmex F-300 (Digitana)

TEST CONCENTRATIONS
- Spacing factor for test concentrations:ca. 3

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

11 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Effect concentrations 2

Duration:

72 h

Dose descriptor:

EC10

Effect conc.:

0.3 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Effect concentrations 3

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

9.8 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

biomass

Effect concentrations 4

Duration:

72 h

Dose descriptor:

EC10

Effect conc.:

2.4 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

biomass