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EC number: 239-349-0 | CAS number: 15307-93-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- other information
- Study period:
- January 17 - February 15, 1995
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: see 'Remark'
- Remarks:
- Well documented and reported study, conducted equivalent to internationally accepted technical guideline in recognized research organization. Although IC and TC values of the test substance at test start are not indicated, the study is regarded as valid because CO2 evolution from endogenous respiration was as expected and degradation of the reference substance was as expected in the absence and presence of the test substance. Therefore, any influence of inorganic carbon can be excluded.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 995
- Report date:
- 1995
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- Version / remarks:
- , 1992
- Deviations:
- yes
- Remarks:
- : Only one CO2 scrubber was used
- GLP compliance:
- yes
Test material
- Reference substance name:
- 2,6-dichloro-N-phenylaniline
- EC Number:
- 239-349-0
- EC Name:
- 2,6-dichloro-N-phenylaniline
- Cas Number:
- 15307-93-4
- Molecular formula:
- C12H9Cl2N
- IUPAC Name:
- 2,6-dichloro-N-phenylaniline
- Details on test material:
- Identification Code (as cited in study report): PBS 3247
Generic/Trade name: Dichlordiphenylamin dest.
BatchNo.: EN 322165.36
Appearance: beige solid
Purity: not available*
Solubility (in water): 0.0063 g/l
Received: 17/10/94
Storage: room temperature
Stability : 12/95
Theoretical carbon content: 60.53 % (purity of 100% assumed)
*: The test substance is identified by the code number. Identification and characterization is guaranteed by the sponsor. Data for purity were not available or not generated and documented according to the rules of Good Laboratory Practices.
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge (adaptation not specified)
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure):
Activated sludge collected from the sewage treatment plant of CH-4153 Reinach on 21/11/94. The pH after collection was 7.6.
- Preparation of inoculum for exposure: The preparation was carried out according to the method described in the guideline.
- Pretreatment: Before application, the inoculum was pre acclimated to the test medium over night. The aeration was performed inside 1.2 l water, containing the amount of mineral salts for 1.5 l. - Duration of test (contact time):
- 29 d
Initial test substance concentrationopen allclose all
- Initial conc.:
- 24.9 - 26.4 mg/L
- Based on:
- test mat.
- Initial conc.:
- 15.1 - 16 mg/L
- Based on:
- other: ThOC
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- TEST CONDITIONS
- Composition of medium: The test medium was prepared according to the method described in the guideline.
- Solubilising agent (type and concentration if used): TWEEN 80, 3 mg/l
- Application of the test substance: Pre acclimated inoculum (1.2 l, see details on inoculum) was made up to 1.5 l with a 300 ml mixture made from: the test substance in 285 ml bidistilled water and 15 ml (4.5 mg) vehicle Polyoxyethylen - Sorbitan monooleate : TWEEN 80 (Fluka 93781), which was applied as a stock solution of 300 mg/l. This mixture was homogenized and added to the test vessel, which was immediately connected to the CO2 scrubber.
- Test temperature: 22 ± 2 °C
- pH: no data
- pH adjusted: no data
- Aeration of dilution water: air purified from carbon dioxide
- Suspended solids concentration: no data
- Continuous darkness: diffuse light (dark brown glass flasks)
TEST SYSTEM
- Culturing apparatus: 2 Liter flasks (dark brown glass) equipped with gas inlet and magnetic stirrer
- Number of culture flasks/concentration: 2
- Method used to create aerobic conditions: air (purified from carbon dioxide) at ca. 25 ml/min
- Measuring equipment: carbon analyzer (Shimadzu TOC-500)
- Test performed in open system: yes
- Details of trap for CO2: Approx 250 ml scrubbers with gas inlet tubes equipped with sintered glass tips containing 200 ml 0.05 N sodium hydroxide. Only one CO2 scrubber was used per test bottle. Theoretically, a solution of 0.05 M NaOH is sufficient to trap at least two times more CO2 than the maximum ThCO2 which can evolve in each test bottle (inc1uding CO2 possibly evolved from the bacteria e.g., endogenous respiration). Moreover, experimentally it was confirmed that no measurable CO2 carry over has ever occurred with the scrubbers used.
SAMPLING
- Sampling frequency:
- blanks and test substance: Day 0, 3, 6, 8, 10, 13, 17, 20, 24, 28, and 29. The last measurement was performed after acidification of the test medium with 1 ml of concentrated hydrochloric acid.
- reference and reference + test substance: Day Day 0, 3, 6, 8, 10, 13, 17, and 20. The specifications of biodegradation criteria were met by that time.
- Sample storage before analysis: samples were analyzed on the day of sampling
CONTROL AND BLANK SYSTEM
- Inoculum blank: test medium and inoculum, 2 flasks
- Abiotic sterile control: not performed
- Toxicity control: reference substance (15 mg DOC/l), test substance (16.5 mg/ThOC/l) and inoculum (1 flask, )
- Procedure control: reference substance (15 mg DOC/l) and inoculum (2 flasks)
Reference substance
- Reference substance:
- benzoic acid, sodium salt
Results and discussion
% Degradation
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 0
- Sampling time:
- 29 d
- Details on results:
- The test substance had no inhibitory effect on the biodegradation of the reference substance (biodegradation (based on the amount of reference item alone) was 58% within 3 days of exposure).
BOD5 / COD results
- Results with reference substance:
- The reference substance sodium benzoate was degraded by 61% within 3 days of exposure.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- Criteria of OECD Guideline 301B for the reference and test substance and the blanks were fulfilled. IC and TC values of the test substance at test start are not indicated. However, the study is regarded as valid (see rationale for reliability).
- Interpretation of results:
- under test conditions no biodegradation observed
- Executive summary:
- The ready degradability of PBS 3247 was determined in a 28-d biodegradation study in accordance with OECD Guideline 301B (Determination of ‘ready’ biodegradability: carbon dioxide (CO2) evolution test (modified Sturm test)). The study was performed in compliance with GLP. Reliability grade 1 was assigned. Tested concentrations were 15.1 and 16.0 mg/l related to ThOC. Microbial activity was not inhibited. PBS 3247 was not readily biodegradable.
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