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Diss Factsheets

Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

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Administrative data

Link to relevant study record(s)

Description of key information

Conclusions are based on read-across from analogue substances of a known category. Acute toxicity to aquatic plants (Algae) (EL50) for 2,2-bis[[(2-ethyl-1-oxohexyl)oxy]methyl]propane-1,3-diyl bis(2-ethylhexanoate)(EC# 230-743-8) is expected to be > 100 mg/l, i.e. >> water solubility.

Key value for chemical safety assessment

EC50 for freshwater algae:
100 mg/L

Additional information

Read across to structural analogue. All members of this category of esters have a hydrophobic nature. Based on structural similarities of the substance of interest and the members of the category it was concluded that it was justified to applyread across for this endpoint.


Selenastrum capricornutum, Fresh Water Algal Growth Inhibition Test with HATCOL 3331.

A limit test was performed exposing exponentially growing algal cultures to a filtered (ca. 5 μm) HATCOL 3331 solution prepared at a loading rate of 100 mg/I and a blank-control. The initial cell density was 104cells/mL. The total test period was 72 hours. Samples for determination of actual exposure concentrations were taken at the start, after 24 hours of exposure and at the end of the test period.


Analysis of the samples showed that average exposure concentrations were at or above the solubility limit of HATCOL 3331 (i.e. <0.2 mg/I).

HATCOL 3331 induced no inhibition of cell growth or reduction of growth rate in Selenastrum capricornutum at the concentration obtained in a filtered solution prepared at a loading rate of 100 mg/l, corresponding to an average exposure concentration of 0.22-0.79 mg/I (NOEC).

Due to the very low solubility of HATCOL 3331 in water, concentration levels that might be toxic for algae could not be reached.