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EC number: 204-402-9 | CAS number: 120-51-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 15/3/1993 - 19/3/1993
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.1 (Acute Toxicity for Fish)
- Deviations:
- yes
- Remarks:
- Temperature decrease greater than 1oC
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- Due to the semi-static nature of this test concentrations were measured in new test solutions and in used test solutions when they were being replaced (every 24 hours).
- Vehicle:
- no
- Details on test solutions:
- Test solutions were created by dissolving a directly weighed amount of the test substance in water using an ultra-turrax. Nominal test concentrations were 0 (control), 3.9, 5.5, 7.8 and 11 mg/l.
- Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- Species: Zebrafish (Brachydanio rerio)
Origin: West-aquarium (Bad Lauterberg)
Date of birth: 12/09/1992
Date of delivery: 26/11/1992
Body length: 2.5-3.5 cm
Pre-test holding: synthethic freshwater according to ISO - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Hardness:
- 14.2 dH
- Test temperature:
- 21.4-22.7
- pH:
- 7.2-8.0
- Dissolved oxygen:
- 7.8-9.7 mg/l
- Salinity:
- Not applicable
- Nominal and measured concentrations:
- Nominal concentrations: 0 (control), 3.9, 5.5, 7.8, 11 mg/l
- Details on test conditions:
- See methods below
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 2.32 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC0
- Effect conc.:
- 1.9 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC100
- Effect conc.:
- 2.84 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- The 96-hour geometric mean of the LC0/LC100 was found to be 0.29 mg/l. Following discussion with the German authority for environmental affairs (Umweltbundesamt) the test results were based on the arithmetic mean from the beginning and end of all exposure times. Based on this the LC0/LC100 was calculated to be 2.32 mg/l. This is considered to be equivalent to the LC50. As such the LC50 can be considered to be 2.32 mg/l.
- Results with reference substance (positive control):
- No information provided
- Reported statistics and error estimates:
- No information provided
- Sublethal observations / clinical signs:
Table 1: Cumulative mortality (%) of fish at all test concentrations and observation points.
Test concentration (mg/l) Cumulative mortality (%) 2 hours Cumulative mortality (%)24 hours Cumulative mortality (%) 48 hours Cumulative mortality (%)72 hours Cumulative mortality (%)96 hours 0 (control) 0 0 0 0 0 3.9 0 0 0 0 0 5.5 0 30 60 100 7.8 0 90 100 11 0 100 - Validity criteria fulfilled:
- not specified
- Conclusions:
- The 96-hour geometric mean of the LC0/LC100 was found to be 0.29 mg/l. This is considered to be equivalent to the LC50. As such the LC50 can be considered to be 0.29 mg/l.
- Executive summary:
A 96 hour acute fish toxicity study was carried out in accordance with EU method C1. Brachydanio rerio were exposed to the test substance at concentrations of 0 (control), 3.9, 5.5, 7.8 and 11 mg/l. A semi-static regime was used and test media was changed every 24 hours. Temperature, pH and dissolved oxygen concentration was measured in new and used media. The 96-hour geometric mean of the LC0/LC100 was found to be 0.29 mg/l. Following discussion with the German authority for environmental affairs (Umweltbundesamt) the test results were based on the arithmetic mean from the beginning and end of all exposure times. Based on this the LC0/LC100 was calculated to be 2.32 mg/l. This is considered to be equivalent to the LC50. As such the LC50 can be considered to be 2.32 mg/l.
Reference
Description of key information
In a 96-hour acute toxicity study the LC50 of benzyl benzoate to Brachydanio rerio was determined to be 2.32 mg/L.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Dose descriptor:
- LC50
- Effect concentration:
- 2.32 mg/L
Additional information
A 96 hour acute fish toxicity study was carried out in accordance with EU method C1.Brachydanio rerio were exposed to the test substance at concentrations of 0 (control), 3.9, 5.5, 7.8 and 11 mg/l. A semi-static regime was used and test media was changed every 24 hours. Temperature, pH and dissolved oxygen concentration was measured in new and used media. The 96-hour geometric mean of the LC0/LC100 was found to be 0.29 mg/l. Following discussion with the German authority for environmental affairs (Umweltbundesamt) the test results were based on the arithmetic mean from the beginning and end of all exposure times. Based on this the LC0/LC100 was calculated to be 2.32 mg/l. This is considered to be equivalent to the LC50. As such the LC50 can be considered to be 2.32 mg/l.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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