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EC number: 293-014-3 | CAS number: 91032-08-5
mean OD550of the replicates (blank-corrected)
total mean OD550of 3 replicate tissues (blank-corrected)
relative tissue viability [%]
SD tissue viability [%]***
CV [% viability]
mean relative tissue viability [%]
* Corrected mean OD550of the negative control corresponds to 100% absolute tissue viability.
** mean relative tissue viability of the three positive control tissues is < 20%
*** standard deviation (SD) obtained from the three concurrently tested tissues is < 18%
In the in vitro skin irritation test using the EpiDerm human epidermis model 25 mg test item + 25 µL DPBS were applied topically for 60 minutes. After 42 h post-incubation cytotoxic effects were determined via MTT reduction assay.
The test item showed no irritant effects. The mean relative tissue viability (% negative control) was> 50% (137%) after 60 min treatment and 42 h post incubation.
This study is classified as acceptable:
Mean OD550 of the three negative control tissues is >=1 and <=2.5.
Mean relative tissue viability of the three positive control tissues is <= 20%.
Standard deviation (SD) of relative tissue viablity obtained from each three concurrently tested tissues is < 18%.
This study satisfies the requirement for Test Guideline OECD 439 for in vitro skin irritation.
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