Iron oxide

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

migrated information: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: see 'Remark'

Remarks:

GLP guideline study. Iron(II)Oxide belongs to the iron oxides family and presents comparable physico chemical properties, leading to similar behaviour for the all members of the iron oxide substances. In particular, based on affinity of the chemical structure, Iron Oxide (Fe3O4) and Iron(III)Oxide (Fe2O3) are selected to support read across for the Iron(II)Oxide dossier. Full justification paper is provided in the current Summary Section.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2000

Report date:

2000

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Triiron tetraoxide

EC Number:

215-277-5

EC Name:

Triiron tetraoxide

Cas Number:

1317-61-9

Molecular formula:

Fe3O4

IUPAC Name:

Magnetite

Details on test material:

Bayferrox 350 liquid
ca. 50 % Iron oxide black (pigment) in aqueous suspension

Sampling and analysis

Analytical monitoring:

no

Test solutions

Details on test solutions:

PREPARATION of STOCK SOLUTION
- 10000 mg/l of the test substance was directly weighed into water, treated for 60 seconds at 8000 rpm with an ultra turrax and stirred for 24 hours on a magnetic stirrer. To remove undissolved particles, the resulting emulsion was filtered using a folded filter of the pore size 7-12 µm.
DILUTION WATER
- Holding water: M4-Medium according to Elendt and BGA (1992)

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

- Females
- Source/supplier: cloned at the Federal Health Office (BGA) in Berlin
- Breeding method: Laboratory bred by parthenogenesis at Bayer AG Leverkusen
- Age: 0 - 24 hours
- Feeding during test: None

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Test conditions

Hardness:

14.6 °dH

Test temperature:

20.1 - 20.5 °C

pH:

7.8

Dissolved oxygen:

8.3 - 8.8 mg/l

Details on test conditions:

Holding water: M4-Medium according to Elendt and BGA (1992)

Results and discussion

Effect concentrations

Duration:

48 h

Dose descriptor:

other: EL0 (= Effective Loading)

Effect conc.:

>= 10 000 mg/L

Conc. based on:

test mat.

Basis for effect:

mobility

Any other information on results incl. tables

Considering the heterogeneous composition of the test substance,
 a Water Accommodated Fraction (WAF) of Bayferrox 350 liquid was tested

Applicant's summary and conclusion

Executive summary:

For triiron tetraoxide a 48-hour EL0 (effective loading) of >= 10000 mg/l (nominal concentration) was observed on Daphnia magna. The method used was EU Method C.2 (Acute Toxicity for Daphnia), (Bayer AG 2000).