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EC number: 201-553-2 | CAS number: 84-69-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Basic toxicokinetics
Administrative data
- Endpoint:
- basic toxicokinetics
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Early non-guideline non-GLP study.
Data source
Reference
- Reference Type:
- publication
- Title:
- Hydrolysis of phthalate esters by purified rat and human liver carboxyesterases
- Author:
- Mentlein, R and Butte, W
- Year:
- 1 989
- Bibliographic source:
- Biochem Pharmacol 38, 3126-3128
Materials and methods
- Objective of study:
- metabolism
Test guideline
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- Three carboxyesterases isolated from rat liver (pI 5.6; pI 6.0; pI 6.2/6.4) and one from human liver. Enzymatic hydrolysis of diisobutyl phthalate (5 mM) was determined with 100 ul of purified enzyme (pH 8.0, 37 degrees C; pH-stat method).
- GLP compliance:
- not specified
Test material
- Reference substance name:
- Diisobutyl phthalate
- EC Number:
- 201-553-2
- EC Name:
- Diisobutyl phthalate
- Cas Number:
- 84-69-5
- Molecular formula:
- C16H22O4
- IUPAC Name:
- 1,2-bis(2-methylpropyl) benzene-1,2-dicarboxylate
- Details on test material:
- - Name of test material (as cited in study report): diisobutyl phthalate, purity not stated, supplied by BASF
Constituent 1
- Radiolabelling:
- no
Test animals
- Species:
- other: rat, human
Results and discussion
Metabolite characterisation studies
- Details on metabolites:
- Hydrolysis of DIBP by human liver carboxyesterase (1.2 umol/min/mg protein) was intermediate between that of 2 rat liver carboxyesterases (0.7-7.4 umol/min/mg protein). Hydrolysis of other dialkyl phthalates also demonstrated but no metabolism of monophthalate esters found.
Any other information on results incl. tables
Hydrolysis of diisobutyl phthalate by human liver carboxyesterase = 1.2 umol/min/mg protein Hydrolysis of diisobutyl phthalate by rat liver carboxyesterase: - carboxyesterase pI 5.6 = 0.7 umol/min/mg protein - carboxyesterase pI 6.2/6.4 = 7.4 umol/min/mg protein - carboxyesterase pI 6.0 not tested Hydrolysis of other dialkyl phthalates (e.g. dimethyl, diethyl, diallyl, dibutyl etc) was also confirmed in these studies however no metabolism of monophthalate esters (e.g. monomethyl, monoethyl) was detected. The authors conclude that liver esterases hydrolyse only one ester bond present in phalate diesters.
Applicant's summary and conclusion
- Conclusions:
- Rat and human that liver carboxyesterases hydrolyse only one of the two ester bonds present in phthalate diesters i.e. convert diisobutyl phthalate to monoisobutyl phthalate.
- Executive summary:
Enzymatic hydrolysis of diisobutyl phthalate by carboxyesterase purified from rat or human liver microsomal fractions was investigated in vitro using a pH-stat method. The highly purified enzymes were confirmed to have acceptable activity toward the model substrates phenyl butyrate (rat isozymes) and methyl butyrate / 4-nitrophenyl acetate (human enzyme). The human and rat liver carboxyesterases exhibited clear activity toward diisobutyl phthalate and other other dialkyl phthalates included in these experiments (e.g. dimethyl, diethyl, diallyl, dibutyl etc), however no metabolism of monophthalate esters (e.g. monomethyl, monoethyl) was found. The authors conclude that liver carboxyesterases hydrolyse only one of the two ester bonds present in phthalate diesters.
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