Methyl chloroacetate

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: No guideline was followed and no GLP. However, the test method is similar to OECD 401.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1979

Report date:

1979

Materials and methods

Principles of method if other than guideline:

No guideline was followed but the test method was similar to OECD 401.

GLP compliance:

no

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Methyl chloroacetate

EC Number:

202-501-1

EC Name:

Methyl chloroacetate

Cas Number:

96-34-4

Molecular formula:

C3H5ClO2

IUPAC Name:

methyl 2-chloroacetate

Details on test material:

- Name of test material (as cited in study report): monochloressigsäuremethylester
- Physical state: colourless liquid

Test animals

Species:

rat

Strain:

Wistar

Sex:

female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Weight at study initiation: 219 ± 24.69 g
- Diet (e.g. ad libitum): Altromin 1324 (Altromin GmbH, Lage/Lippe), ad libitum
- Water (e.g. ad libitum): tap water, ad libitum

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

other: sesame oil

Details on oral exposure:

VEHICLE
- Concentration in vehicle: 0.5 g substance/100 ml vehicle

Doses:

50, 80, 100, 125, 200 and 315 mg/kg bw

No. of animals per sex per dose:

10 females per dose

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: body weight was recorded at 0, 7 and 14 days
- Other examinations performed: clinical signs, necropsy.

Statistics:

Probit analysis

Results and discussion

Effect levels

Sex:

female

Dose descriptor:

LD50

Effect level:

107 mg/kg bw

95% CL:

97.2 - 121

Mortality:

Mortality was observed at 100, 125, 200 and 315 mg/kg bw.

Clinical signs:

other: Passivity, disturbances of equilibrium, prone position, piloerection, diarrhea, laboured respiration.

Gross pathology:

Liver, spleen and kidneys deep brown coloured and strongly supplied with blood, red ovaries.

Any other information on results incl. tables

Table 7.2.1 -1: Mortality

Dose (mg/kg bw)	Mortality
50	0/10
80	0/10
100	4/10
125	8/10
200	10/10
315	10/10

 

Applicant's summary and conclusion

Interpretation of results:

Toxicity Category III

Remarks:

Migrated information Criteria used for interpretation of results: EU

Conclusions:

The oral LD50 (females) was 107 mg/kg bw with a 95% confidence interval between 97.2 and 121 mg/kg bw.

Executive summary:

The test substance methyl chloroacetate was administered to groups of female Wistar rats by oral gavage at the doses of 50, 80, 100, 125, 200 and 315 mg/kg body weight. Animals were subjected to daily observations and weekly determinations of body weight. Macroscopic examinations were performed on the animals found dead. Mortality was observed at 100, 125, 200 and 315 mg/kg bw. The oral LD50 was 107 mg/kg bw with a 95% confidence interval between 97.2 and 121 mg/kg bw.