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Ecotoxicological information

Short-term toxicity to fish

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Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment
Justification for type of information:
Study not to standard test method or to GLP, but sufficient detail available to allow assessment.

Data source

Reference
Reference Type:
publication
Title:
Metal Bioaccumulation Patterns in Major Carps during Acute Toxicity Tests
Author:
ABDULLAH, S et al
Year:
2011
Bibliographic source:
International Journal of Agriculture & Biology;Oct2011, Vol. 13 Issue 5, p756

Materials and methods

Principles of method if other than guideline:
juvenile fish are exposed for specified period of time e.g., 96-h to a range of toxicant concentrations in a static system.
GLP compliance:
not specified

Test material

Reference
Name:
Unnamed
Type:
Constituent

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
At the end of each test, water samples were taken and analyzed for the corresponding metal concentration by following the methods as described in S.M.E.W.W. (1989). The analytical data obtained confirmed that the determined concentrations in the test mediums coincided with the estimated data quite satisfactorily. The concentrations of each metal in the fish (whole body) before and after acute toxicity tests were determined (S.M.E.W.W., 1989).

Test organisms

Test organisms (species):
other: Catla catla, Labeo rohita and Cirrhina mrigala
Details on test organisms:
TEST ORGANISM
- Source:Fish stock (induced bred) of desired weight and age were obtained from the Fish Seed Hatchery, Faisalabad, Pakistan.
- Age: 30 days, 60 days and 90 days
- Food and feeding: Fish were fed with crumbled feed (35% digestible protein & 2.90 kcal g-1 digestible energy) during adaptations, but they were not fed during the last 24 h of adaptations and throughout the test duration.


ACCLIMATION
- Acclimation period: 14 days

Study design

Test type:
static
Water media type:
freshwater
Total exposure duration:
96 h

Test conditions

Hardness:
100 mg/L.
To maintain the total hardness, CaSO4 and MgSO4 were used to to increase hardness, while ethylenediaminetetraacetic acid and its sodium salts
(EDTA) were used to decrease water hardness when required.
Test temperature:
30oC
Dissolved oxygen:
During all the trials, constant air was supplied to the test mediums with air pumped through a capillary system.
Details on test conditions:
TEST SYSTEM
All glassware and aquariums used in this experiment were washed with nitric acid and thoroughly rinsed with deionized water prior to use.
Prior to each trial, all aquariums (70-L capacity) were filled with 50-L dechlorinated tap water of desired hardness (100 mg L-1) and pH (7).
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 3
:
OTHER TEST CONDITIONS
- Adjustment of pH: NaOH and HCl were added as required to maintain pH
- Photoperiod: 12 hours

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Dead fish were weighed individually after being lightly blotted dry at the time of mortality observations. No mortality was observed among control fish.

TEST CONCENTRATIONS
The concentrations tested for three fish species, separately, were started from zero with an increment of 0.05 and 5 mg L-1 (as total concentration) for low and high concentrations, respectively.

Results and discussion

Effect concentrations
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
>= 55.26 - <= 67.71 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
element
Basis for effect:
mortality
Reported statistics and error estimates:
Statistical analyses were performed with SPSS 10.1 Computer program. Differences in metal toxicity means among species were analyzed by Analysis of Variance and Duncan’s Multiple Range tests (Steel et al., 1996). Relationships among various parameters were determined from Pearson Correlation Coefficients.

Any other information on results incl. tables

Mean LC50 and lethal concentrations of three fish age groups

Fish Species

LC50 concentrations (±SE) (mgL-1)

Lethal concentrations (±SE) (mgL-1)

 

30-day

60-day

 90-day

30-day

 60-day

90-day

Catla catla

55.26 ± 3.67

64.67 ± 3.61

67.71 ± 3.94

92.03 ± 7.20

99.59 ± 6.52

107.72±6.83b

Labeo rohita

64.13 ± 3.39

71.15 ± 3.77

73.70 ± 3.64

99.82 ± 6.63

110.54 ±7.04

108.74±6.44

Cirrhina mrigala

71.24 ± 3.87

76.23 ± 4.55

91.68 ± 4.65

110.43± 6.81

123.21± 8.11

142.13±9.66

 

Mean metals concentrations in three age groups of fish before and after acute exposure

 

30-day

 60-day

 90-day

 

Before exposure

After exposure

Before exposure

After exposure

Before exposure

After exposure

Catla catla

8.19 ± 0.21

61.61 ± 2.32

10.39±0.43

158.79± 11.11

11.11 ± 0.23

160.12 ± 1.23

Labeo rohita

8.62 ± 0.36

67.39 ± 3.99

11.65±0.52

168.42±42.43

11.69 ± 0.58

168.88 ± 2.52

Cirrhina mrigala

8.73 ± 0.69

79.15 ± 1.79

12.84±0.63

183.39±23.46

13.15 ± 0.36

186.15 ± 1.95

Applicant's summary and conclusion

Conclusions:
The 96 h LC50 for Catla catla (Indian carp) was reported as 55-68 mg/L