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EC number: 233-828-8 | CAS number: 10377-66-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP and conducted to current guideline. In addition, manganese nitrate has been shown to be stable at the concentration and pH used in the study (refer to IUCLID section 5.6) supporting the high reliability of the result.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 010
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- manganese dinitrate tetrahydrate
- IUPAC Name:
- manganese dinitrate tetrahydrate
- Details on test material:
- - Molecular formula (if other than submission substance): Mn(NO3)2.4H20
- Physical state: solid
- Stability under test conditions: Stable
- Storage condition of test material: approximately 4°C over silica gel in the dark
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 100 mg/l
- Sampling method: The test samples were analysed following addition of nitric acid (1 ml per 20 ml of sample or equivalent).
- Sample storage conditions before analysis: not stored
Test solutions
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: the test item was dissolved directly in reconstituted water. An amount of test item (100 mg) was dissolved in reconstituted water and the volume adjusted to 1 litre to give the 100 mg/l test concentration from which serial dilutions were prepared in reconstituted water to give the remainder of the test series of 10, 1.0, 0.10 and 0.010 mg/l. The prepared concentration was inverted several times to ensure adequate mixing and homogeneity
- Controls: The control group was maintained under identical conditions but not exposed to the test item
- Evidence of undissolved material (e.g. precipitate, surface film, etc): no undissolved material was observed.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna
- Strain: 1st instar
- Source: in-house laboratory cultures
- Age at study initiation : less than 24 hours old
- Feeding during test: no
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- 250 mg/l as CaCO3
- Test temperature:
- 20°C
- pH:
- 7.7-7.8
- Dissolved oxygen:
- 8.7-9.0 mg/l
- Salinity:
- Not reported
- Nominal and measured concentrations:
- Nominal
Sample Concentration Nominal Concentration Concentration of Manganese Expressed as a Percentage
(mg/l) of manganese Found (mg/l) of the Manganese Concentration (%)
0 Hours Control R1 - R4 -100 R1 - R2 22 21.4 97
100 R3 - R4 22 21.4 97
48 Hours Control R1 - R4 -100 R1 - R2 22 20.2 92
100 R3 - R4 22 20.8 94 - Details on test conditions:
- TEST SYSTEM
- Test vessel: glass jars
- Type : closed
- Material, size, headspace, fill volume: 250 ml
- Aeration: none
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: The dilution water was reconstituted water prepared by addition of an aliquot(25 ml) of the following solutions to each litre of deionised water with a conductivity of < 5µS cm-1:
a) CaCl2.2H2O 11.76 g/l
b) MgSO4.7H2O 4.93 g/l
c) NaHCO3 2.59 g/l
d) KCl 0.23 g/l
- Intervals of water quality measurement: 0, 24 and 48 hrs
OTHER TEST CONDITIONS
- Adjustment of pH: The reconstituted water had a pH of 7.8 ± 0.2 adjusted (if necessary) with NaOH or HCl initially, but not during the test.
- Photoperiod: 16 hours light and 8 hours darkness cycle with 20 minute dawn and dusk transition periods.
EFFECT PARAMETERS MEASURED : Immobilisaton based on being unable to swim for approximately 15 seconds after gentle agitation.
TEST CONCENTRATIONS
- Spacing factor for test concentrations: not applicable as limit test at 100 mg/l
- Range finding study: yes
- Test concentrations in range-finding study: 100, 10, 1.0, 0.10 and 0.010 mg/l
- Results used to determine the conditions for the definitive study: yes - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
Results and discussion
Effect concentrations
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- element
- Remarks:
- manganese was measured by ICP-MS and concentration related back to test material.
- Basis for effect:
- mobility
- Remarks:
- If daphnia did not swim for approximately 15 seconds after gentle agitation.
- Details on results:
- - Behavioural abnormalities: none
- Mortality of control: none
- Other adverse effects control: none
- Abnormal responses: none
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: none
- Effect concentrations exceeding solubility of substance in test medium: no - Results with reference substance (positive control):
- - Results with reference substance valid? Yes
- EC50: 0.77 mg/l - Reported statistics and error estimates:
- An estimate of the EC50 values was given by inspection of the immobilisation data.
For the positive control The EC50 values and associated confidence limits at 24 hours and the slope of the response curve and standard error were calculated by the maximum-likelihood probit method (Finney 1971) using the ToxCalc computer software package (ToxCalc 1999). The EC50 value and associated confidence limits at 48 hours were calculated by the trimmed Spearman-Karber method (Hamilton et al 1977) using ToxCalc computer software package (ToxCalc 1999).
Probit analysis is used where two or more partial responses to exposure are shown.
When only one potential response is shown the trimmed Spearman-Karber method is appropriate.
Any other information on results incl. tables
Cumulative Immobilisation Data in the Definitive Test
Nominal |
Cumulative Immobilised Daphnia |
||||||
24 Hours |
48 Hours |
||||||
No. Per Replicate |
Total |
% |
No. Per Replicate |
Total |
% |
||
Control |
R1 |
0 |
0 |
0 |
0 |
0 |
0 |
|
R2 |
0 |
0 |
||||
|
R3 |
0 |
0 |
||||
|
R4 |
0 |
0 |
||||
100 |
R1 |
0 |
0 |
0 |
0 |
0 |
0 |
|
R2 |
0 |
0 |
||||
|
R3 |
0 |
0 |
||||
|
R4 |
0 |
0 |
R1– R4= Replicates 1 to 4
Cumulative Immobilisation Data in the Positive Control
Nominal |
Cumulative Immobilised Daphnia |
|||||||
24 Hours |
48 Hours |
|||||||
R1 |
R2 |
Total |
% |
R1 |
R2 |
Total |
% |
|
Control |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0.32 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0.56 |
1 |
1 |
2 |
10 |
3 |
2 |
5 |
25 |
1.0 |
7 |
7 |
14 |
70 |
10 |
10 |
20 |
100 |
1.8 |
10 |
10 |
20 |
100 |
10 |
10 |
20 |
100 |
3.2 |
10 |
10 |
20 |
100 |
10 |
10 |
20 |
100 |
Verification of test concentrations
Sample |
Nominal Concentration (mg/l) |
Nominal Concentration of manganese |
Concentration of Manganese Found (mg/l) |
Expressed as a Percentage of the Manganese Concentration (%) |
0 Hours |
Control R1- R4 |
- |
<LOQ |
- |
|
100 R1- R2 |
22 |
21.4 |
97 |
|
100 R3- R4 |
22 |
21.4 |
97 |
48 Hours |
Control R1- R4 |
- |
<LOQ |
- |
|
100 R1- R2 |
22 |
20.2 |
92 |
|
100 R3- R4 |
22 |
20.8 |
94 |
LOQ = Limit of quantitation
R1– R4= Replicates 1 to 4
Physico-Chemical Measurements
Nominal |
0 Hours |
24 Hours |
48 Hours |
|||||||
pH |
mg O2/l |
%* |
T°C |
TºC |
pH |
mg O2/l |
%* |
T°C |
||
Control |
R1 |
8.0 |
9.0 |
99 |
20 |
20 |
8.0 |
8.8 |
97 |
20 |
|
R2 |
8.0 |
9.0 |
99 |
20 |
20 |
8.0 |
8.7 |
96 |
20 |
|
R3 |
8.0 |
9.0 |
99 |
20 |
20 |
8.0 |
8.8 |
97 |
20 |
|
R4 |
8.0 |
9.0 |
99 |
20 |
20 |
8.0 |
8.7 |
96 |
20 |
100
|
R1 |
7.8 |
9.0 |
99 |
20 |
20 |
7.7 |
8.7 |
96 |
20 |
R2 |
7.8 |
9.0 |
99 |
20 |
20 |
7.7 |
8.7 |
96 |
20 |
|
R3 |
7.8 |
9.0 |
99 |
20 |
20 |
7.8 |
8.7 |
96 |
20 |
|
R4 |
7.8 |
9.0 |
99 |
20 |
20 |
7.8 |
8.7 |
96 |
20 |
*= Dissolved oxygen concentration expressed as a percentage of Air Saturation Value
R1– R4= Replicates 1 to 4
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- Manganese dinitrate tetrahydrate is not acutely harmful to daphnia magna, with an EC(50) > 100 mg/l.
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