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EC number: 203-640-0 | CAS number: 109-02-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Study period:
- 1981
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- GLP compliance:
- no
- Specific details on test material used for the study:
- - Name of test material (as cited in study report): 4-methylmorpholine
- Analytical monitoring:
- no
- Vehicle:
- no
- Test organisms (species):
- activated sludge, industrial
- Details on inoculum:
- - Source: activated sludge of an wwtp treating industrial sewage (BASF AG)
- Initial biomass concentration: dry matter: 1 g/L - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 30 min
- Test temperature:
- ambient temperature
- Nominal and measured concentrations:
- nominal: 15, 150, 2000 mg/L
- Key result
- Duration:
- 30 min
- Dose descriptor:
- other: EC20
- Effect conc.:
- > 2 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Validity criteria fulfilled:
- not specified
- Conclusions:
- In this study, the EC20 (30min) was concluded to be > 2000 mg/L.
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Study period:
- 1991
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Qualifier:
- according to guideline
- Guideline:
- ISO 8192 (Water quality - Test for inhibition of oxygen consumption by activated sludge for carbonaceous and ammonium oxidation)
- Qualifier:
- according to guideline
- Guideline:
- other: EC Directive 88/302/EEC Activated sludge: Testing of respiration inhibition
- GLP compliance:
- no
- Specific details on test material used for the study:
- - Name of test material (as cited in study report): 4-methylmorpholine
- Substance type: pure active substance
- Physical state: liquid
- Analytical purity: >= 99% - Analytical monitoring:
- no
- Vehicle:
- no
- Test organisms (species):
- activated sludge, domestic
- Details on inoculum:
- - Source: activated sludge of a laboratory scale wwtp treating domestic and synthetic sewage
- Initial biomass concentration: dry matter: 1 g/L - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 30 min
- Test temperature:
- ambient temperature
- Nominal and measured concentrations:
- nominal: 1000 mg/L (related to TOC: 574 mg/L)
- Details on test conditions:
- - test volume: 250 mL
- Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorphenol (3,5-DCP); concentrations: 4 and 40 mg/L (1.86 and 18.6 mg/L related to TOC)
- Key result
- Duration:
- 30 min
- Dose descriptor:
- other: EC20
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Validity criteria fulfilled:
- not specified
- Conclusions:
- In this study, the EC20 (30min) was concluded to be > 1000 mg/L.
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Study period:
- No data
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: see 'Remark'
- Remarks:
- Test performed according to a non-standard method (Pseudomonas putida O2 consumption test), which was demonstrated to yield similar results (for a series of test substances) as the Pseudomonas putida growth inhibition test (DIN 38 412, part 8). The test results can be considered reliable with restrictions, because not all details were reported in the publication.
- Qualifier:
- no guideline available
- Principles of method if other than guideline:
- Pseudomonas putida O2 consumption test (5-6 h incubation, O2 levels tested at start and end of exposure period).
- GLP compliance:
- no
- Specific details on test material used for the study:
- - Name: N-methylmorpholin
- No further details reported - Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- see test conditions
- Test organisms (species):
- Pseudomonas putida
- Details on inoculum:
- - Laboratory culture: yes
- Method of cultivation: cultured on agar (incubated at 25°C), each 4-6 weeks transferred to fresh agar (containing D-glucose, NaNO3, K2HPO4, KH2PO4, MgSO4.6H2O, iron citrate, trace elements, vitamins)
- Preparation of inoculum for exposure: For inoculation of pre-cultures, 7-d old cultures were used. Pre-cultures (100 mL pre-culture medium in 250-mL closed Erlenmeyer flasks) were inoculated with 2 mL inoculum from the 7-d-old cultures and incubated for 19 h (continuous shaking). Pre-culture medium contained D-glucose, NaNO3, K2HPO4, KH2PO4, FeSO4.7H2O, MgSO4.6H2O, trace element solution, and water.
- Initial cell density: ca. 1E+06 cells/mL (cell density in pre-cultures measured photometrically at 436 nm) - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 6 h
- Remarks on exposure duration:
- 5-6 h, depending on O2 levels of blank controls (minimal O2 level in blank controls (without HgCl2) should be 3.0-3.5 mg O2/L - this was checked from 4.5 h on and as soon as these levels were attained the test was finished).
- Hardness:
- not applicable
- Test temperature:
- 25°C
- pH:
- not reported
- Dissolved oxygen:
- not applicable
- Salinity:
- not applicable
- Nominal and measured concentrations:
- Control + a series of test substance concentrations <= 1000 mg/L. Test concentrations not reported.
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 100-mL sterilized glass bottles, fill-volume 100 mL, no headspace
- Type (delete if not applicable): closed
- Aeration: no
- Initial cell density: ca. 1E+06 cells/mL
- No. of vessels: 4 per test concentration (of which 2 with HgCl2), 4 blank controls with HgCl2, 5 blank controls without HgCl2
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: bidistilled water
- Test medium contained D-glucose, NaNO3, K2HPO4, KH2PO4, FeSO4.7H2O, MgSO4.6H2O, trace element solution.
- Culture medium different from test medium: yes
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: not reported
- Light intensity: not reported
- Continuous stirring by magnetic stirrer
EFFECT PARAMETERS MEASURED (with observation intervals if applicable):
- O2 consumption was calculated based on measurements of O2 levels at start and end of testing.
- Test vessels were ended by addition of 1 mL HgCl2 solution (300 mg/L) to stop bacterial growth.
- O2 levels were measured using an O2 electrode.
TEST CONCENTRATIONS
- Spacing factor for test concentrations: not reported - Reference substance (positive control):
- no
- Key result
- Duration:
- 6 h
- Dose descriptor:
- EC10
- Effect conc.:
- 95 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: O2 consumption
- Remarks on result:
- other: mean of 3 tests
- Details on results:
- For a series of other substances, the outcome of the method was compared to that of the Pseudomonas putida growth inhibition test (DIN 38 412, part 8), resulting in good correlation (R2 = 0.982 and 0.983 for EC10 and EC50 values, respectively).
- Reported statistics and error estimates:
- EC10 and EC50 were determined using graphical methods or Probit analysis.
- Validity criteria fulfilled:
- yes
- Conclusions:
- In this study, the oxygen consumption in closed bottles inoculated with Pseudomonas putida was measured at the start and at the end of a 5 to 6 hour exposure period and compared to the oxygen consumption in blank controls. Three replicate tests yielded an average 6-h EC10 of 95 mg/L.
Referenceopen allclose all
- blank value (mean of two blanks): 15 mg 02/g dry matter
Concentration |
Respiration rate |
Change of respiration rate |
(mg/L) |
(mg O2/L*h) |
(%) |
15 |
14 |
-7 |
150 |
16 |
+7 |
2000 |
17 |
+13 |
No significant inhibition ot the respiration was observed with the
test substance up to a concentration of 2000 mg/L. A stimulation rather
than an inhibition of the respiration was observed.
- blank value (mean): 29 mg 02/g dry matter
Concentration |
Respiration rate |
Change of respiration rate |
(mg/L) |
(mg O2/L*h) |
(%) |
1000 |
28 |
-3 |
Reference substance: 4 |
7 |
-76 |
Reference substance: 40 |
24 |
-17 |
No significant inhibition ot the respiration was observed with the
test substance at a concentration of 1001 mg/l.
Description of key information
Three Klimisch 2 studies are available and taken forward in a weight-of-evidence approach. Given the presence of deviations in all three studies, the OECD 209-test result from BASF (1991-04-02) was chosen to derive PNEC-STP and not a test result which was not performed according to standardized test method. The respiration inhibition test from BASF was performed according to a guideline valid at that time including a test duration of 30 min. The 30 min. EC20 value of > 1000 mg/L is used for the CSA and derivation of the PNEC.
Key value for chemical safety assessment
- EC10 or NOEC for microorganisms:
- 1 000 mg/L
Additional information
A weight-of-evidence approach is applied to cover this endpoint.
The respiration inhibition study (OECD 209) by BASF AG (1981) reported a 30-min EC20 of >2000 mg/L. Three concentrations were tested (15, 150 and 2000 mg/L) using industrial sewage sludge from a BASF WWTP. There is no mentioning of any possible adaptation of the sludge. The EC10 value is not mentioned either, but the test results indicate that it lies between 150 en 2000 mg/L.
The respiration inhibition study (OECD 209) by BASF AG (1991) reported a 30-min EC20 of >1000 mg/L. The single concentration tested was 1000 mg/L using domestic/synthetic sewage sludge. The EC10 value is not mentioned but extrapolation from the data shows it is >1000 mg/L.
Both BASF studies tested for 30 minutes, while 3 hours is the standard according to OECD TG 209.
The Pseudomonas putida oxygen consumption test is not a standard test normally used for this endpoint either. However, the publication (Schöberl, 1995) reported a close similarity with results of the Pseudomonas putida growth inhibition test (DIN 38 412, part 8). The 6h-EC10 value was 95 mg/L.
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