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EC number: 400-390-6 | CAS number: 87787-67-5 FLEXSORB-SE
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- July 2001
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Study conducted in accordance with generally accepted scientific principles, possibly with incomplete reporting or methodological deficiencies, which do not affect the quality of relevant results.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- no
- GLP compliance:
- not specified
- Analytical monitoring:
- yes
- Details on sampling:
- For each test concentration, after a 3 hour incubation period the respiration rates were measured in each vessel.
- Details on test solutions:
- Nominal test concentrations (mg/l) of: 0 (control), 3.2, 10, 32, 100, 320, 1000 were prepared.
- Test organisms (species):
- activated sludge, domestic
- Details on inoculum:
- Activated sludge, freshly obtained from a sewage treatment plant receiving largely domestic sewage, used as the microbial source.
- Test type:
- static
- Water media type:
- not specified
- Limit test:
- no
- Total exposure duration:
- 3 h
- Post exposure observation period:
- After a 3 hour incubation period the concentration of dissolved oxygen (mg/l) was measured every minute in each vessel for 10 minutes.
- Hardness:
- Not known.
- Test temperature:
- The test was performed at a temperature of 19.0ºC
- pH:
- Not known.
- Dissolved oxygen:
- The concentration of dissolved oxygen (mg/l) was measured every minute in each vessel for 10 minutes (after 3 hour incubation).
- Salinity:
- Not known.
- Nominal and measured concentrations:
- Nominal test conentrations (mg/l): 0 (control), 3.2, 10, 32, 100, 320, 1000
- Details on test conditions:
- See table below for test treatments.
The reference stock (0.5 g/l) was prepared in deionised water as described in OECD test guideline 209.
The appropriate volume of dilution water was measured into each test vessel and vigorously aerated. At time zero the required volume of synthetic sewage feed and activated sludge was added to the dechlorinated tap water in the first flask. The appropriate amount of test material was added (if required) and the aeration restored. This process was repeated at 15 minute intervals for the remaining flasks, using the volumes previously indicated.
After a 3 hour incubation period the respiration rates were measured in each vessel as follows. The contents were poured into 250ml glass BOD bottles and the oxygen electrode inserted. The probe displaced liquid and thus any air from the measuring apparatus. The contents were stirred continuously using the probes built in stirrer and the concentration of dissolved oxygen (mg/l) was measured every minute in each vessel for 10 minutes. The test was performed at a temperature of 19.0ºC - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol (3,5-DCP)
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- >= 320 - <= 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- dissolved
- Remarks:
- oxygen
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Details on results:
- Between 3.2 and 100 mg/l test substance there was no apparent dose related response. However, for 320 mg/l and 1000 mg/l a response was observed with 18 and 63% inhibition respectively. The data clearly indicates that the EC50 lies between 320 mg/l and 1000 mg/l although an exact value could not be calculated with so few data.
The EC80 and EC20 values of the test substance, equally, could not be calculated.
The results of the abiotic control flask (test without addition of inocula) indicated that there would be no reduction in oxygen concentration in any of the test vessels up to, and including, 1000 mg/l of the test substance, attributable to processes in the test system other than those due to the activity of the activated sludge. - Results with reference substance (positive control):
- The EC50 for the reference substance 3,5-DCP was 5.62 mg/l, satisfying the OECD validity condition that the EC50 for this substance be in the range 5 to 30 mg/l. The second validity condition, given in OECD 209, that the two control flasks had respiration rates within 15% of each other was also satisfied in this test.
- Validity criteria fulfilled:
- yes
- Conclusions:
- Between 3.2 and 100 mg/l of the test substance there was no apparent dose related response. However, for 320 mg/l and 1000 mg/l a response was observed with 18 and 63% inhibition respectively. The data clearly indicates that the EC50 lies between 320 mg/l and 1000 mg/l although an exact value could not be calculated with so few data.
The EC80 and EC20 values of the test substance, equally, could not be calculated. - Executive summary:
This report describes a study to determine the effect of the test substance on activated sludge micro-organisms by measuring the respiration rate, under defined conditions, in the presence of different concentrations of the test substance. The method is based on that described in OECD test guideline 209 in which activated sludge, freshly obtained from a sewage treatment plant receiving largely domestic sewage, is used as the microbial source. The purpose of the study is to assess the possible effect of the test substance on aerobic microbial treatment plants and to indicate suitable non-inhibitory concentrations of the test substance for biodegradability tests.
Two controls, without test substance, were included in the study to determine the degree of experimental variation. The sensitivity of the activated sludge was estimated using three concentrations of the reference substance 3,5-dichlorophenol (3,5-DCP).
Between 3.2 and 100 mg/l of the test substance there was no apparent dose related response. However, for 320 mg/l and 1000 mg/l a response was observed with 18 and 63% inhibition respectively. The data clearly indicates that the EC50 lies between 320 mg/l and 1000 mg/l although an exact value could not be calculated with so few data.
The EC50 for the reference substance 3,5-DCP was 5.62 mg/l, satisfying the OECD validity condition that the EC50 for this substance be in the range 5 to 30 mg/l. The second validty conition, given in OECD 209, that the two control flasks had respiration rates within 15% of each other was also satisfied in this test.
Reference
Treatment |
Respiration rate (R) (mg O2/l/hr) |
Percentage respiration inhibition (I) |
Control |
30.51 |
|
Abiotic 1000 mg/l |
0.40 |
99 |
DCP 5 mg/l |
15.99 |
48 |
DCP 15 mg/l |
9.02 |
71 |
DCP 30 mg/l |
3.96 |
87 |
3.2 mg/l |
32.24 |
-5 |
10 mg/l |
32.82 |
-7 |
32 mg/l |
32.20 |
-5 |
100 mg/l |
31.05 |
-1 |
320 mg/l |
25.14 |
18 |
1000 mg/l |
11.45 |
63 |
Control |
30.74 |
|
Description of key information
ASRIT NOEC = 100 mg/L; OECD 209; Chemex (2001)
Key value for chemical safety assessment
- EC50 for microorganisms:
- 320 mg/L
- EC10 or NOEC for microorganisms:
- 100 mg/L
Additional information
In an activated sludge respiration inhibition test conducted in accordance with OECD 209, no effect was seen on 3h respiration inhibition between 3.2 and 100 mg/l, resulting in a NOEC of 100 mg/L.. Inhibition of respiration was observed between 320 mg/l and 1000 mg/l ashowing 18 and 63% inhibition respectively. The report defined the EC50 between 320 mg/l and 1000 mg/l.
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