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Short-term toxicity to fish

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short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
comparable to guideline study with acceptable restrictions
equivalent or similar to
OECD Guideline 203 (Fish, Acute Toxicity Test)
Solubility not determined, actual concentrations not measured, just nominal reported. Appendix and tables missing information such as pH, hardness, etc. Fish not fed 55 hours before test instead of 24 hours. No deviations exist that affected the scientif
GLP compliance:
Analytical monitoring:
Details on test solutions:
- Appropriate quantities of the test substance were weighed, added to appropriate volumes of well water and stirred for 2 hours to create individual test solutions. Stability and solubility in dilution water and under storage conditions were not determined.
- Undissolved test substance was observed in all test solutions.
Test organisms (species):
Pimephales promelas
Details on test organisms:
- Common name: Fathead minnow
- Length at study initiation: 1.3-2.2 cm standard length and 0.004 to 0.099 g for controls, data not provided for treated fish.
- Feeding during test
- Amount: No food 55 hours prior to and during test

- Acclimation period: Reared from egg to size used for test
- Acclimation conditions (same as test or not): Yes for temperature
- Type and amount of food: Artemia sp. (newly hatched)
- Feeding frequency: Twice daily on weekdays and daily on weekends
- Health during acclimation (any mortality observed): No sickness, injury, or abnormality observed
Test type:
Water media type:
Limit test:
Total exposure duration:
96 h
Hardness and alkalinity within expected ranges
Test temperature:
21.5-21.7 degrees C
Declined over the duration of the test according to the magnitude of the test concentration down to 3.5
Dissolved oxygen:
Within expected ranges
Nominal and measured concentrations:
Rangefinding concentrations: 0.1, 1.0, 10, 100, and 1000 mg/L.
Nominal test concentrations: 78, 130, 216, 360, 600, and 1000 mg/L
Details on test conditions:
- Test vessel:
- Type: open
- Material, size, headspace, fill volume: Glass aquaria of 20 litres, (39(L) x 20(W) x 24 (H)) containing 15L of test solution (approximately 19-cm liquid depth)
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): Not specified
- Biomass loading rate: 0.035 g/L for control fish
- Photoperiod of 16 hours light (108-366 Lux): 8 hours darkness employed with 25 minutes of transitional light (approximately 2.2 Lux) preceding and following the 16 hour light interval.

- Source/preparation of dilution water: well water
- Intervals of water quality measurement: daily for DO, pH, and temperature

- Adjustment of pH: no
- Photoperiod:16 hours light vs. 8 hours darkness with 25 minutes of transitional light
- Light intensity: 108-377 Lux. Transitional light was 2.2 Lux.

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Cumulative mortality and behavioural data

- Range finding study
- Test concentrations: 0.1, 1.0, 10, 100, and 1000 mg/L
Reference substance (positive control):
96 h
Dose descriptor:
Effect conc.:
133.7 mg/L
Nominal / measured:
Conc. based on:
test mat.
Basis for effect:
Remarks on result:
other: 95% fiducial limit = 116.7-154.9 mg/L
Details on results:
Range-finding test with 5 fish per concentration was conducted with a water control. Mortalities were 0,0,0,0,0 and 100% respectively.
For main test, mortality increased as test solution increased and pH decreased with total kill observed at the 4 highest test concentrations (216, 360, 600, and 100 mg/L), between pH 3.5 and 5.1.
Reported statistics and error estimates:
95% fiducial interval of 96-hr LC50 = 116.7 to 154.9 mg/L

Authors state that low pH effects probably accounted for mortalities at 4 highest test concentrations to a greater degree than direct toxicity of the test material. However, since the pH was within the expected range at the 96 -hour LC50 nominal concentration (unadjusted for undissolved test substance), the observed mortality was probably attributable to inherent compound toxicity.

Validity criteria fulfilled:
This study established a 96-hour LC50 in fathead minnows of 133.7 mg/L with a 95% fiducial interval of 116.7 to 154.9 mg/L (nominal concentrations).
Executive summary:

Isophthaloyl dichloride exhibited slight toxicity in a 96 -hour, unaerated, static, acute test using fathead minnows, Pimephales promelas. Nominal test substance concentrations were 78, 130, 216, 360, 600, and 1000 mg/L. The 96-hour LC50 was 133.7 mg/L with a 95% fiducial interval of 116.7 to 154.9 mg/L.

Description of key information

The study was conducted similar to OECD 203 under GLP with Fathead minnows,Pimephales promelas. The 96-hour, unaerated, static study used nominal test concentrations of 78, 130, 216, 360, 600 and 1000 mg/L. The test substance exhibited slight toxicity with a LC50of 133.7 mg/L.

Key value for chemical safety assessment

LC50 for freshwater fish:
133.7 mg/L

Additional information