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Environmental fate & pathways

Biodegradation in water: screening tests

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Description of key information

Results:
a. OECD Guideline 302 C: 82% biodegradation after 28 d (inherently biodegradable)
b. OECD Guideline 301 D: 16% biodegradation after 28 d (not readily biodegradable)
b. OECD Guideline 302 C: 82% biodegradation after 28 d (inherently biodegradable)

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Additional information

The inherent biodegradation of tributyl acetyl citrate was investigated in a study conducted corresponding to OECD Guideline 302C: "Inherent Biodegradabilty: Modified MITI Test (II)"over a period of 28 days and using activated sludge as inoculum. The biodegradation rate was determined by measurement of oxygen consumption. Inoculum blank and procedural/functional control with the reference substance aniline were performed.The test item proved to be inherently biodegradable under the test conditions employed (82% biodegradation after 28 d). The functional control reached the pass level >60% after 14 d.

The ready biodegradation of tributyl acetyl citrate was investigated in a study conducted according to EU Method C.4-E (Determination of the "Ready" Biodegradability - Closed Bottle Test; 2008) and OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test; 1992) over a period of 28 days and using a mixed inoculum composed of sewage from a sewage treatment plant working predominantly on domestic sewage and soil. The biodegradation rate was determined by measurement of oxygen consumption. Inoculum blank, procedural/functional control with the reference substance sodium benzoate, and toxicity control using 1.642 mg/L test substance and 3.033 mg/L reference compound were performed. The test item proved to be not readily biodegradable under the test conditions employed (16% biodegradation after 28 d). The functional control reached the pass level >60% after 14 d. In the toxicity control containing both test and reference item 47% biodegradation based on ThOD occurred within 14 d thus indicating that the test item was not inhibitory at the concentration tested.