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Environmental fate & pathways

Bioaccumulation: aquatic / sediment

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Administrative data

Endpoint:
bioaccumulation in aquatic species: fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
16 April to 07 May 2003
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Study undertaken at GLP accredited laboratory to internationally accepted guidelines.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2003
Report Date:
2003

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 305 (Bioconcentration: Flow-through Fish Test)
Deviations:
no
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
solid: particulate/powder
Remarks:
migrated information: powder
Details on test material:
- Name of test material (as cited in study report): BPS-MAE
- Physical state: White powder
- Analytical purity: 99.2%
- Lot/batch No.: G130131
- Expiration date of the lot/batch:
- Stability under test conditions:
- Storage condition of test material: Desiccator at room temperature.
- Other: Date of receipt: 14 February 2003
Radiolabelling:
no

Sampling and analysis

Details on sampling:
- Sampling intervals/frequency for test organisms:On days 5, 7, 14, 21 and 28.
- Sampling intervals/frequency for test medium samples: On days 0, 5, 7, 14, 21 and 28.
- Sample storage conditions before analysis: no data
- Details on sampling and analysis of test organisms and test media samples (e.g. sample preparation, analytical methods):
From a acetonitrile (HPLC reagent grade) solution containing 1000mg/L the test substance, 0.2 mL was taken and added to a pair of control fish, which was then analyzed to evaluate recovery. Added to a fishweight of 10 g, the test substance would exist at 20 pg/g, which is 100 times of the high concentration level (0.2 mg/L). The four measurements of recovery gave a mean value of 91.6%, a standard deviation of 0.7% and a range of 1.3%, which is satisfactory for quantitative analysis. The average recovery was used to correct the measured values.

Test solutions

Vehicle:
yes
Details on preparation of test solutions, spiked fish food or sediment:
Identity and concentration of auxiliary solvent for dispersal:
2-methoxyethanol = 25 ppm (v/v) used for high concentration level (0.2 mg//l), low concentration level (0.02 mg/l) and control.

Test organisms

Test organisms (species):
Cyprinus carpio
Details on test organisms:
TEST ORGANISM
- Common name: Carp
- Source: Commercial fish supplier
- Age at study initiation: < 1 year
- Length at study initiation: 8 ± 4 cm
- Weight at study initiation: approx 5 g
- Weight at termination (mean and range, SD):
- Method of breeding: no data
- Health status: good
- Description of housing/holding area: 56l glass aquarium with 50l of water.
- Feeding during test
- Food type: Solution
- Amount: 20 ml
- Frequency: daily

ACCLIMATION
- Acclimation period: >= 2 weeks
- Acclimation conditions: same as test
- Type and amount of food: Babygold from Kyorin; The food was given in an amount of 2% of the fish weight.
- Feeding frequency: daily except week-ends.
- Health during acclimation (any mortality observed): < 5%

Study design

Route of exposure:
aqueous
Test type:
flow-through
Water / sediment media type:
natural water: freshwater
Total exposure / uptake duration:
7 - 28 d
Total depuration duration:
0 d

Test conditions

Hardness:
73 mg/l CaCO3
Test temperature:
Test (solution) temperature: 24 °C (min.) ... 26 °C (max.)
pH:
Test (solution) pH: 6 (min.) ... 8.5 (max.)
Dissolved oxygen:
>= 60% of the saturation
Details on test conditions:
Duration of test: 28 d
Nominal and measured concentrations:
Concentration of test material used: 0.02 amd 0.2 mg/L
Reference substance (positive control):
no

Results and discussion

Lipid contentopen allclose all
Lipid content:
6.5 - 7.7 %
Time point:
start of exposure
Lipid content:
5.7 - 6.8 %
Time point:
end of exposure
Bioaccumulation factoropen allclose all
Type:
BCF
Value:
>= 16 - <= 24 dimensionless
Basis:
whole body w.w.
Calculation basis:
steady state
Remarks on result:
other: Conc.in environment / dose:0.02 mg/l
Type:
BCF
Value:
>= 3 - <= 8 dimensionless
Basis:
whole body w.w.
Calculation basis:
steady state
Remarks on result:
other: Conc.in environment / dose:0.2 mg/l
Depuration
Depuration time (DT):
0 min
Details on results:
- Mortality of test organisms: none
- Behavioural abnormalities: none
- Observations on body length and weight: no data
- Organ specific bioaccumulation: no data
- Mortality and/or behavioural abnormalities of control: none
- Loss of test substance during test period:no data

Applicant's summary and conclusion

Validity criteria fulfilled:
not specified
Conclusions:
On the basis of the BCF obtained, it can be concluded that the possibility for fish to bioaccumulate the test substance is slight under the prescribed test condition.