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EC number: 230-804-9 | CAS number: 7327-60-8
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- April-May 2001
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Well conducted and reported study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 001
- Report date:
- 2001
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 870.1100 (Acute Oral Toxicity)
- GLP compliance:
- yes
- Test type:
- acute toxic class method
- Limit test:
- no
Test material
- Reference substance name:
- Nitrilotriacetonitrile
- EC Number:
- 230-804-9
- EC Name:
- Nitrilotriacetonitrile
- Cas Number:
- 7327-60-8
- Molecular formula:
- C6H6N4
- IUPAC Name:
- 2-[bis(cyanomethyl)amino]acetonitrile
- Details on test material:
- Batch no: 3554B
Date of receipt: 3 April 2001
Purity: 95 wt%
Moisture: 5 wt%
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Sprague-Dawley
- Sex:
- male/female
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- corn oil
- Details on oral exposure:
- The test material was mixed with corn oil; 10 mg/mL for the low dose, 40 mg/mL for the high dose.
Single gavage application at 5 mL/kg bw. - Doses:
- 50 and 200 mg/kg bw
- No. of animals per sex per dose:
- 5
- Control animals:
- no
- Details on study design:
- Duration of observation period following administration: 14 days
Frequency of observations and weighing: several times on the day of treatment and once daily thereafter
Necropsy of survivors performed: yes
Other examinations performed: body weight (weekly) - Statistics:
- Not applicable
Results and discussion
Effect levels
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- > 50 - < 200 mg/kg bw
- Based on:
- test mat.
- Mortality:
- None at 50 mg/kg bw
Four males and two females (60%) at 200 mg/kg bw. - Clinical signs:
- other: At 50 mg/kg bw: staggered gait, wet/dark-stained urogenital area on the day of treatment At 200 mg/kg bw: hypoactivity, staggered gait, clonic convulsions, wet area around the mouth, red-stained face, lacrimation, wet urogenital area. In surviving animals
- Gross pathology:
- Only in animals that died (200 mg/kg bw); changes consisted of moist, red oral discharge.
Applicant's summary and conclusion
- Interpretation of results:
- Category 3 based on GHS criteria
- Remarks:
- Migrated information
- Conclusions:
- The oral LD50 was between 50 and 200 mg/kg bw (close to 200 mg/kg bw).
- Executive summary:
An acute oral toxicity study was carried out in Sprague Dawley rats. Two groups of 5 male and 5 female rats each were dosed by single gavage at 50 or 200 mg/kg bw and kept for 14 days observation. None of the rats dosed with 50 mg/kg died; 60% mortality was observed at 200 mg/kg bw. Therefore the LD50 was between 50 and 200 mg/kg bw, close to 200 mg/kg bw.
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