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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Description of key information

One GLP study and one non-GLP study conducted according to OECD guidelines 203 are available for the 2-nitropropane.
The key study determined a 48-hour EC50 for 2-nitropropane of 19 mg 2-nitropropane/L in the aquatic invertebrate, Daphnia magna. The supporting study determined a 24-hour EC50 for 2 nitropropane of 290 mg 2-nitropropane in the aquatic invertebrate, Daphnia magna.

Key value for chemical safety assessment

EC50/LC50 for freshwater invertebrates:
19 mg/L

Additional information

The key study was conducted recently, had analytical confirmation of test solution concentrations and was conducted under good laboratory practice (GLP) principles. Because of this it is determined to be high quality. The exposure (48-hour) was conducted using a flow-through system that allow frequent turnover of the volume of test solutions in test vessels. Analytical confirmation of test solution concentrations during the test confirmed that target concentrations were achieved and maintained adequately throughout the 48-hour exposure. The EC50 based on daphnid immobility was determined to be 19 mg/L with a NOEC of 5.9 mg/L. The supporting study was not conducted under GLP principles. The study report lacked detail but provided enough that it could be considered acceptable to provide supporting evidence with reservations. The exposure period in this study was 24 hours rather than 48 hours as in the key study. In addition, it was not conducted flow-through or with sealed vessels so volatility may have been a factor. This study determined an EC50 based on daphnid immobility of 290 mg/L. 

 

Based on this information, the more recentDaphnia magnaacute test is considered the key study for the short-term toxicity to aquatic invertebrates endpoint. The EC50 of 19 mg/L would result in a classification of Harmful for this material based on daphnid toxicity.