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Environmental fate & pathways

Biodegradation in water: screening tests

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Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
May 1995
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: GLP compliant, guideline study, available as unpublished report, limitations in reporting but otherwise adequate for assessment.
Justification for type of information:
A discussion and report on the read across strategy is given as an attachment in Section 13.
Cross-reference
Reason / purpose:
read-across: supporting information
Reference
Endpoint:
biodegradation in water: ready biodegradability
Type of information:
read-across based on grouping of substances (category approach)
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: GLP compliant, guideline study, available as unpublished report, limitations in reporting but otherwise adequate for assessment.
Justification for type of information:
A discussion and report on the read across strategy is given as an attachment in Section 13.
Reason / purpose:
read-across source
Qualifier:
according to
Guideline:
OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
GLP compliance:
yes
Remarks:
No certificate
Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, domestic (adaptation not specified)
Duration of test (contact time):
28 d
Initial conc.:
37.54 mg/L
Based on:
test mat.
Parameter followed for biodegradation estimation:
O2 consumption
Reference substance:
benzoic acid, sodium salt
Parameter:
other: ThOD
Value:
> 74.1 - < 80
Sampling time:
28 d
Details on results:
10% biodegradation reached on day 3, 60.9 and 62.9% degradation on day 11.
Results with reference substance:
Reference substance showed 84.8% biodegradation after 28 days.
Validity criteria fulfilled:
yes
Interpretation of results:
readily biodegradable
Conclusions:
n-olefins C11-12 were readily biodegradable in an OECD 301F
Executive summary:

This GLP study was conducted to standard guidelines. The analytical method used to determine oxygen consumption is not stated in the report. Despite this limitation the validity criteria of the test are met and so this study is considered suitable for use. n-olefins C11 -C12 are considered readily biodegradable as a mean of 77.1% degradation occurred within 28 days.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1995
Report Date:
1995

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
GLP compliance:
yes
Remarks:
No certificate

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
mix of n-undecene and n-dodecene, colourless liquid
Specific details on test material used for the study:
Details on properties of test surrogate or analogue material (migrated information):
Not reported

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge (adaptation not specified)
Details on inoculum:
obtained from the aeration basin of the municipal water treatment plant, Novara, Italy. Sludge aerated at room temperature for 24 hours prior to the test.
Duration of test (contact time):
28 d
Initial test substance concentration
Initial conc.:
37.54 mg/L
Based on:
test mat.
Parameter followed for biodegradation estimation
Parameter followed for biodegradation estimation:
O2 consumption
Details on study design:
Inoculum concentration of 30mg/l suspended solids. Test conducted at 22±0.2°C. As the test substance is insoluble in water itwas added directly to the test flasks by a microsyringe. Duplicate test and blank controls were run and the oxygen consumption measured daily.
Reference substance
Reference substance:
benzoic acid, sodium salt

Results and discussion

Preliminary study:
data not reported
Test performance:
data not reported
% Degradation
Parameter:
other: ThOD
Value:
> 74.1 - < 80
Sampling time:
28 d
Details on results:
10% biodegradation reached on day 3, 60.9 and 62.9% degradation on day 11.

BOD5 / COD results

Results with reference substance:
Reference substance showed 84.8% biodegradation after 28 days.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Interpretation of results:
readily biodegradable
Conclusions:
n-olefins C11-12 were readily biodegradable in an OECD 301F
Executive summary:

This GLP study was conducted to standard guidelines. The analytical method used to determine oxygen consumption is not stated in the report. Despite this limitation the validity criteria of the test are met and so this study is considered suitable for use. n-olefins C11 -C12 are considered readily biodegradable as a mean of 77.1% degradation occured within 28 days.