3-(difluoromethyl)-1-methyl-1H-pyrazole-4-carboxylic acid

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2007-10-11 to 2007-10-18

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study with analytical monitoring

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2008

Report date:

2008

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

- Sampling method: At the start of the exposure (0 h) samples from vessels without daphnids and at the end of the exposure (48 h) samples from vessels with daphnids were taken.

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: The test solution of 120 mg/L was prepared by dissolving 120.1 mg of test substance with 1000 mL test medium. The solution was treated with a ultrasonic bath for approx. 3 minutes, then the solution was stirred on a magnetic stir plate for about 10 minutes at approximately 20 ± 2 °C.
- Evidence of undissolved material: None

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Water flea
- Source: Originally obtained from the Institut National de Recherche Chimique Appliquée, France, in 1978. From this date on this clone was cultured and bred continuously in the Laboratory of Ecotoxicology of the Experimental Toxicology and Ecology of the BASF SE, Ludwigshafen, Germany.
- Age at study initiation: < 24h
- Method of breeding: Photoperiod: 16 hours light, 8 hours dark, temperature: 20 ± 2 °C, diet: Desmodesmus subspicatus, feeding: Daily, Renewal of the breeding water: Twice weekly
- Feeding during test: None

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Test conditions

Hardness:

2.20 - 3.20 mmol/L

Test temperature:

20.3 - 21.1 °C

pH:

7.5 - 8.5

Dissolved oxygen:

8.8 - 9.0 mg/L

Salinity:

550 - 650 µS/cm

Nominal and measured concentrations:

Nominal: 0 (blank) and 120 mg/L
Measured: 124.9 (0h) and 122.0 (48h) mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: Test tubes (glass) with flat bottom (nominal volume 20 mL)
- Fill volume: 10 mL
- Aeration: None

- No. of organisms per vessel: 5
- No. of vessels per concentration: 4
- No. of vessels per control: 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: According to guideline
- Acid capacity up to pH 4.3: 0.80 - 1.00 mmol/L
- Ca/mg ratio: about 4:1
- Conductivity: 550-650 µS/cm
- Intervals of water quality measurement: At test start and at test ending

OTHER TEST CONDITIONS
- Photoperiod: 16 hours day : 8 hours night
- Light intensity: 20 – 850 Lux at a wave length of 400 - 750 nm

EFFECT PARAMETERS MEASURED: Immobility

Reference substance (positive control):

yes

Remarks:

Potassium dichromate

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

>= 120 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 120 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 3

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

> 120 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

- Mortality of control: 0

Results with reference substance (positive control):

- Results with reference substance valid? Yes
- Mortality: The EC50(24h) of the control substance potassium dichromate was 1.17 mg/L (experimental conduct in accordance with GLP but without a GLP Status, conducted on 13 Sep 2007, project number: 50E0063/043039). This result is within the range of 0.6 – 2.1 mg/L (ISO 6341).