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EC number: 204-624-6 | CAS number: 123-39-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Skin sensitisation
Administrative data
- Endpoint:
- skin sensitisation
- Remarks:
- in vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- not reported
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Comparable to guideline study with acceptable restrictions.
Data source
Reference
- Reference Type:
- publication
- Title:
- Intralaboratory validation of alternative endpoints in the murine local lymph node assay for the identification of contact allergic potential: primary ear skin irritation and ear-draining lymph node hyperplasia induced by topical chemicals.
- Author:
- Ulrich, P. et al.
- Year:
- 2 001
- Bibliographic source:
- Arch. Toxicol. 74, 733-744, 2001
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 406 (Skin Sensitisation)
- Deviations:
- yes
- Principles of method if other than guideline:
- Method: other: according to Ulrich, P. et al.: Toxicology 125, 149-168
- GLP compliance:
- not specified
- Type of study:
- mouse local lymph node assay (LLNA)
Test material
- Reference substance name:
- Automatically generated during migration to IUCLID 6, no data available
- IUPAC Name:
- Automatically generated during migration to IUCLID 6, no data available
- Details on test material:
- N,N-dimethylformamide, no further data
Constituent 1
In vivo test system
Test animals
- Species:
- mouse
- Strain:
- Balb/c
- Sex:
- female
- Details on test animals and environmental conditions:
- 6-8 weeks old
Study design: in vivo (LLNA)
- Vehicle:
- acetone/olive oil (4:1 v/v)
- Remarks:
- DMF was a vehicle for other chemicals
- No. of animals per dose:
- 6
- Details on study design:
- ANIMAL ASSIGNMENT AND TREATMENT
Name of test method: two.tired murine local lymph node assay
Results and discussion
In vivo (LLNA)
Results
- Parameter:
- SI
- Remarks on result:
- other: First experiment: slight ear-draining lymph node activation as expressed by increased weights and cell counts. However, this observation was not reproducible in a second experiment.
Any other information on results incl. tables
RS-Freetext:
Topical treatment of mice with the vehicle
N,N-dimethylformamide led to slight ear-draining lymph node
activation as expressed by increased weights and cell counts
in comparison to the untreated animals.
However, this observation was not reproducible in a second
experiment (i.e. when DMF was tested as vehicle for eugenol
and as vehicle alone in comparison to the respective
untreated control group).
Applicant's summary and conclusion
- Interpretation of results:
- not sensitising
- Remarks:
- Migrated information
- Conclusions:
- Topical treatment of mice with the vehicle N,N-dimethylformamide led to slight ear-draining lymphnode activation as expressed by increased weights and cell counts in comparison to the untreated animals. However, this observation was not reproducible in a second experiment
- Executive summary:
N,N-dimethylformamide was used as a vehicle in the present
investigation.
The publication describes the validation of a two-tiered
murine local lymph node assay.
Groups of 6 female BALB/C strain mice (6-8 weeks old) were
used. During tier I a wide range of concentrations of test
chemical solutions or vehicle (volume: 25 µl) were applied
on three consecutive days to the dorsum of both ears. Mice
were killed 24 hours after the last application to determine
ear and local lymph node weights and lymph node cell counts.
Ear weights were determined to correlate chemical induced
skin irritation with the ear-draining lymph node activation
potential.
For comparison of the induction and challenge responses,
mice were treated on the shaved back with 50 µl of test
chemical or vehicle alone on three consecutive days
(induction phase treatment). Then mice were challenged 12
days after the final induction phase exposure with 25 µl of
test chemical or vehicel on the dorsum of both ears for a
further 3 days (challenge phase treatment). Lymph nodes were
excised 24 hours after the final challenge phase treatment.
A tier II LLNA protocol was used to finally differentiate
between true irritants and contact allergens.
To investigate the impact of different vehicles on the
primary response induced by two contact allergens, i.e
dinitrochlorobenzene (DNCB) at 0.5% and by eugenol at 35%,
DAE433, DMSO, DMF and acetone /oil olive (AOO) were used.
Both contact allergens were compared either to the untreated
control (aqua bidest) or to the corresponding vehicle
control.
RM-Freetext:
non-BASF study
RM-Freetext:
relevant for MSDS
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