N-methylformamide

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1990-01-15 to 1990-01-18

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: comparable to guideline study with acceptable restrictions (exposure concentrations in the test and the stability of the test substance were not confirmed by analysis)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1992

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: DIN 38412/9

Principles of method if other than guideline:

Method: other: Scenedesmus-Zellvermehrungshemmtest in Anlehnung an DIN 38412/9; Messung der Biomasseentwicklung

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

N-methylformamide

EC Number:

204-624-6

EC Name:

N-methylformamide

Cas Number:

123-39-7

Molecular formula:

C2H5NO

IUPAC Name:

N-methylformamide

Details on test material:

- Name of test material (as cited in study report): Monomethyl formamide
- Other: Test substance as prescribed by 1.1 - 1.4

Sampling and analysis

Analytical monitoring:

no

Test organisms

Test organisms (species):

Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

72 h

Test conditions

Test temperature:

20 ±1 °C

pH:

8.1 - 8.8

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): open / closed
- Material, size, headspace, fill volume: 250 mL erlenmeyer tube filled with 100 mL test solution
- Initial cells density: 10 000 cells/mL
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4


GROWTH MEDIUM
- Standard medium used: yes according to Din 38412 part 9


OTHER TEST CONDITIONS
- Sterile test conditions: yes
- Photoperiod: permanent illumunation
- Light intensity and quality: 10000 lux


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Chlorophyll measurement: EOS flourometer

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

72 h

Dose descriptor:

EC10

Effect conc.:

> 500 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Effect concentrations 2

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

> 500 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Effect concentrations 3

Duration:

72 h

Dose descriptor:

EC90

Effect conc.:

> 500 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Any other information on results incl. tables

Effect values (endpoint: biomass):

EbC10 (72 h) > 500 mg/L

EbC50 (72 h) > 500 mg/L

EbC90 (72 h) > 500 mg/L 

(values related to nominal concentrations)