Reaction mass of 1,2-Benzenedicarboxylic acid, 4-sulfo-, trisodium salt, 1,2-Benzenedicarboxylic acid, 3-sulfo-, sodium salt (1:3), Sodium sulphate

Toxicity to microorganisms

Administrative data

Endpoint:

toxicity to microorganisms

Type of information:

migrated information: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Non-GLP Guideline study without analytical verification of test concentrations

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Report date:

1987

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

DIN 38412-8 (Pseudomonas Zellvermehrungshemmtest)

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Sulfoisophtalsäure Na-Salz

IUPAC Name:

Sulfoisophtalsäure Na-Salz

Details on test material:

- Name of test material (as cited in study report): Sulfoisophtalsäure Na-Salz

Sampling and analysis

Analytical monitoring:

no

Test solutions

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: dosage based on stock solution
- Differential loading: no
- Controls: 1 inoculated sample
- Evidence of undissolved material (e.g. precipitate, surface film, etc): no

Test organisms

Test organisms (species):

Pseudomonas putida

Details on inoculum:

- Laboratory culture: Pseudomonas putida regularly obtained from DSM (German collection of microorganisms, Goettingen, Germany)
- strain: DSM 50026
- cultured on agar
- Method of cultivation:
- Temperature: 21 ± 1 °C
- Illumination: Day:night rhythm
- nutrient medium: 1.0 g/L Sodium nitrate, 0.24 g/L dipotassium hydrogen phosphate, 0.12 g/L Potassium dihydrogen phosphate, 0.2 g/L magnesium sulphate heptahydrate, 10.0 g/L D(+)-Glucose, 0.0005 g/L ferric citrate, 0.1 g/L yeast extract, 18.0 g/L agar
- Pre-culture:
- Test vessel: Erlenmeyer flasks, volume 250 mL, fill volume 100 mL
- inoculated with bacterial suspension at a turbidity of 10 TE/F at 21 ± 1 °C
- incubation time: 7 hours, shaking
- nutrient medium: 0.5 g/L Sodium nitrate, 0.12 g/L dipotassium hydrogen phosphate, 0.06 g/L Potassium dihydrogen phosphate, 0.2 g/L magnesium sulphate heptahydrate, 2.0 g/L D(+)-Glucose, 0.0005 g/L ferric citrate, 0.05 g/L yeast extract

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

17 h

Test conditions

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Type: Erlenmeyer flask
- Material, fill volume: glass, 300 mL
- Aeration: no
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Biomass loading rate: 1 mL of a Pseudomonas suspension adjusted to a turbidity of 10 TE/F

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: nutrient medium: 0.5 g/L Sodium nitrate, 0.12 g/L dipotassium hydrogen phosphate, 0.06 g/L Potassium dihydrogen phosphate, 0.2 g/L magnesium sulphate heptahydrate, 2.0 g/L D(+)-Glucose, 0.0005 g/L ferric citrate

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Photometric determination of light extinction after 7 hours of exposure

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

7 h

Dose descriptor:

EC10

Effect conc.:

69 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth inhibition

Effect concentrations 2

Duration:

7 h

Dose descriptor:

EC50

Effect conc.:

94 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth inhibition

Effect concentrations 3

Duration:

7 h

Dose descriptor:

other: EC90

Effect conc.:

119 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth inhibition