2,2'-[6-(4-methoxyphenyl)-1,3,5-triazine-2,4-diyl]bis{5-[(2-ethylhexyl)oxy]phenol}

Toxicity to soil microorganisms

Administrative data

Endpoint:

toxicity to soil microorganisms

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2017

Report date:

2017

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 216 (Soil Microorganisms: Nitrogen Transformation Test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: 0015986251
- Expiration date of the lot/batch: 09.09.2021
- Purity test date: not indicated

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: ambient at room temperature
- Stability under test conditions: given

- Purity: 100 %
- water solubility: insoluble

Sampling and analysis

Analytical monitoring:

no

Test substrate

Vehicle:

no

Details on preparation and application of test substrate:

The test substance was added to the test medium directly mixed with quartz sand.

Test organisms

Test organisms (inoculum):

soil

Study design

Total exposure duration:

28 d

Test conditions

Test temperature:

20.8 - 22.3 °C

Moisture:

Water content: 5.1 g/100g dw (mean value of 2 values)

Details on test conditions:

TEST SYSTEM
- Testing facility: BASF SE
- Test container (type, material, size): 2 L glass container with a wide opening, overed with perforated aluminium lid
- Amount of soil: approx. 1147 g
- Amount of quartz sand: approx. 10 g
- No. of replicates per concentration: 1
- No. of replicates per control: 1


SOURCE AND PROPERTIES OF SUBSTRATE (if soil)
Soil type: 2.3
Batch no.: F2.3 0917
Sampling depth: Approximately 20 cm
Sampling quantity: Approximately 1000 kg
Sampling date: 01 Mar 2017
Weather condition on sampling: Rainfall with air temperature of 7 °C
Preparation of the soil by the supplier: Drying for 5 days at room temperature, Presieving
with 10 mm screen, Last sieving 2 mm screen.
Supplier: Landwirtschaftliche Untersuchungs- und Forschungsanstalt (LUFA) Speyer, Obere Langgasse 40, 67346 Speyer, Germany.
Arrival in the testing facility: 09 Mar 2017
Organic carbon: 0.68 ± 0.04%
Nitrogen content: 0.08 ± 0.01%
Sand (particle size >0.063 -2.0 mm): 57.2 ± 2.4 % (German DIN)
Soil type according German DIN: Silty sand (uS)
pH-value (0.01M CaCl2): 5.84 ± 0.6
Cation exchange capacity: 7.5 ± 0.8 meq/100g
Water holding capacity: 35.4 ± 1.0 g/100g
Grain size: 2 mm
Dry weight of the soil (measured): 95.2 % (mean value of 2 values)
Water content of the soil (measured): 5.1 g/100g Dw (mean value of 2 values)
Initial microbiological biomass (measured): 426.4 ± 20.0 mg carbon/kg Dw (mean value of 3 values, correspond to 6.3% of carbon content of the soil)

NITROGEN SOURCE:
Luzerne meal
Supplier: Tock GmbH Futtermühle; 66798 Wallerfangen-Ihn, Weinbachstr. 18-20
Date of delivery: 18 June 2003
Crude protein: 17 %
Carotene: 80 mg/kg
Content of carbon: 41.9 g/100g
Content of nitrogen: 2.4 g/100g
C/N ratio: 17/1

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : measurement of nitrigen content after 7, 14 and 28 d.

VEHICLE CONTROL PERFORMED: no

Nominal and measured concentrations:

Nominal: 0, 62.5, 125, 250, 500, 1000 mg/kg dw

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Key result

Duration:

28 d

Dose descriptor:

EC10

Effect conc.:

>= 1 000 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

nitrate formation rate

Effect concentrations 2

Duration:

28 d

Dose descriptor:

EC50

Effect conc.:

> 1 000 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

nitrate formation rate

Reported statistics and error estimates:

The statistical evaluation of the data was carried out using ToxRat. If the % inhibition was lower than 0 % it was set to 0 %. If the %inhibition was greater than 100 % it was set to 100 %. A curve was fitted between the concentration and the % inhibition via the probit model. This curve was used for the estimation of the EC10, EC25 and EC50. Appropriate weighting for the model fitting via linear transformation were used. The confidence intervals were determined via normal approximation.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

No inhibitory effect son the nitrogen formation up to the highest test concentration of 1000 mg/kg soil dry weight.