Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 272-902-4 | CAS number: 68919-76-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2012-09-24 to 2012-12-20
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: The study has been performed according to OECD guideline 301B and EU method C.4.C in a GLP certified testing facility.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- Deviations:
- yes
- Remarks:
- (Temperature range was 17.1 - 21.5°C instead of 20- 24°C. As degradation of the positive control was in the normal range this is considered as uncritical concerning the outcome of the study)
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-C (Determination of the "Ready" Biodegradability - Carbon Dioxide Evolution Test)
- Deviations:
- yes
- Remarks:
- (Temperature range was 17.1 - 21.5°C instead of 20- 24°C. As degradation of the positive control was in the normal range this is considered as uncritical concerning the outcome of the study)
- GLP compliance:
- yes (incl. QA statement)
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Duration of test (contact time):
- 28 d
- Initial conc.:
- 26.7 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Key result
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 6
- Sampling time:
- 28 d
- Details on results:
- The degree of biodegradation reached 6 % after 28 days.
No 10-day window was detected.
The abiotic degradation reached 1.2%. - Validity criteria fulfilled:
- yes
- Interpretation of results:
- under test conditions no biodegradation observed
- Conclusions:
- The ready biodegradability of 400112 was determined in the CO2 Evolution Test following OECD 301B resp. EU C.4.C.
The degree of biodegradation reached 6 % after 28 days.
No 10-day-window was detected.
The abiotic degradation reached 1.2%.
Therefore the test item 400112 is considered as “not readily biodegradable" - Executive summary:
Ready degradability is defined in the guidelines as degradation surpassing 60% within 10 days after reaching a level of 10%. For the test item 400112 the degree of biodegradation reached 6% after 28 days. A 10-day window was not detected. Therefore, the test item 400112 can not be considered as “readily biodegradable”. Degradation missed 60% within 28 days, too. Therefore, the test item is not considered as “biodegradable within 28 days”, either.
Degradation behaviour of the positive control (Aniline) and the toxicity control was normal. Abiotic degradation was 1.2%. Both replicates of the test item showed good correspondence.
All validity criteria were met. No observations were made which might cause doubts concerning the validity of the study outcome. The result of the test can be considered valid.
Reference
Emitted Carbon in mg/L
Day |
Control 1 |
Control 2 |
Positive Control 1 |
Positive Control 2 |
Test 1 |
Test 2 |
Abiotic Control |
Toxicity Control |
2 |
0.23 |
0.46 |
0.76 |
0.59 |
0.29 |
0.50 |
0.11 |
0.41 |
4 |
0.85 |
1.50 |
5.19 |
2.11 |
1.12 |
1.34 |
0.35 |
3.95 |
7 |
1.24 |
1.90 |
11.00 |
7.90 |
1.81 |
1.92 |
0.18 |
8.68 |
9 |
1.94 |
2.72 |
15.76 |
11.00 |
2.45 |
3.00 |
0.17 |
12.04 |
11 |
2.32 |
2.96 |
17.68 |
12.50 |
2.66 |
3.41 |
0.22 |
13.46 |
14 |
2.60 |
3.61 |
19.34 |
15.41 |
3.06 |
4.04 |
0.16 |
16.63 |
18 |
2.94 |
3.68 |
20.76 |
18.00 |
3.46 |
4.56 |
0.18 |
17.06 |
23 |
2.98 |
3.55 |
20.02 |
18.16 |
3.86 |
4.71 |
0.09 |
16.70 |
29 |
3.67 |
4.20 |
20.28 |
19.37 |
4.48 |
5.77 |
0.24 |
18.20 |
Degradation Values
In the following table, the percentage biodegradation is presented:
Day |
Positive Control 1 |
Positive Control 2 |
Positive Control Mean |
Test 1 |
Test 2 |
Test Mean |
Abiotic Control |
Toxicity Control |
2 |
2.0 |
1.2 |
1.6 |
-0.3 |
0.8 |
0.2 |
0.5 |
0.2 |
4 |
19.6 |
4.6 |
12.1 |
-0.3 |
0.8 |
0.3 |
1.8 |
6.9 |
7 |
46.0 |
30.9 |
38.4 |
1.2 |
1.7 |
1.5 |
0.9 |
17.6 |
9 |
65.5 |
42.3 |
53.9 |
0.6 |
3.3 |
1.9 |
0.9 |
24.0 |
11 |
73.3 |
48.1 |
60.7 |
0.1 |
3.8 |
2.0 |
1.1 |
26.7 |
14 |
79.2 |
60.0 |
69.6 |
-0.2 |
4.6 |
2.2 |
0.8 |
33.4 |
18 |
85.1 |
71.7 |
78.4 |
0.7 |
6.1 |
3.4 |
0.9 |
34.0 |
23 |
81.7 |
72.7 |
77.2 |
2.9 |
7.1 |
5.0 |
0.5 |
33.2 |
29 |
79.7 |
75.3 |
77.5 |
2.7 |
9.1 |
5.9 |
1.2 |
35.2 |
All validity parameters and values are presented in the following table:
Parameter |
Criterion |
Found |
Assessment |
IC content of test item solution in medium |
≤ 5% of TC |
1.72% |
valid |
CO2emitted by the controls |
< 70 mg/L |
14.4 mg/L |
valid |
Difference within replicates |
≤20% |
6.4% |
valid |
Degradation of positive control > 60% |
≤ 14 days |
11 days |
valid |
Degradation in the toxicity flask on day 14 |
> 25% |
33% |
valid |
Description of key information
The ready biodegradability of 400112 was determined in the CO2 Evolution Test following OECD guideline 301B resp. EU C.4.C.
The degree of biodegradation reached 6 % after 28 days.
No 10-day window was detected.
The abiotic degradation reached 1.2%.
Therefore the test item 400112 is considered as “not readily biodegradable"
Key value for chemical safety assessment
- Biodegradation in water:
- under test conditions no biodegradation observed
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.