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Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
November 1989
Reliability:
1 (reliable without restriction)
Justification for type of information:
Experimental test result performed according to the guideline.
Qualifier:
according to
Guideline:
other: EEC-Guideline L251 Vol.27, 84/449/EEC: C.l
Deviations:
no
Principles of method if other than guideline:
The EC50-values were calculated according to SPEARMAN-KAERBER in: D.J. Finney, 524-530, London (1964).EC-values were graphically determined on gausso-logarithmic probability paper.
GLP compliance:
yes
Analytical monitoring:
not specified
Vehicle:
yes
Details on test solutions:
Stock solution: 10 mg TK 11484 were mixed and made up to 1000 ml with test water
Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: Daphnia magna
- Strain: Daphnia magna Straus 1820
- Source: CIBA-GEIGY Ltd., Facilities, Basle
- Age at study initiation (mean and range, SD): 6-i24h of age
- Method of breeding: Cultures of daphnia are maintained in glass vessels containing approx. 2.5 L of reconstituted water (see below) at 20 ± 1°C (water is renewed partially thrice weekly). At each renewal the daphnia are fed a suspension of green algae (Scenedesmus subspicatus) supplemented by a suspension of TETRAMIN-extract in such quantities that the food is consumed after 24h.
- Feeding during test : None during the test
- Number of daphnia : 20 daphnia per concentration and control 4 replicates of 5 daphnia each'
- Pretreatment : ≤24h before the start of the test reproductive daphnia are separated from the young by sieving all individuals through a 800 µm seive. This operation is repeated 6h before the start of the test and the young (6-≤24h of age) are retained for the test.
Test type:
static
Water media type:
freshwater
Total exposure duration:
24 h
Hardness:
240 mg CaCO3/L
Test temperature:
20±1°C
Nominal and measured concentrations:
Test concentrations (mg/l) : 0.0010, 0.0018, 0.0032, 0.0058, 0.010, 0.018, 0.032, 0.058, 0.10, 0.18, 0.32, 0.58, 1.0
Remarks (nominal) : Calculated amounts of stock solution to produce the desired test concentrations were given into the water and were homogeneously distributed. The daphnia were then transfered into the beakers.
Details on test conditions:
TEST SYSTEM
- Test vessel: Beakers covered with watch glasses 100 ml solution per beaker
- Type (delete if not applicable): closed
- Aeration: The water was aerated with clean air for at least 24h before use. No aeration during test

OTHER TEST CONDITIONS
- Adjustment of pH:
- Photoperiod: Fluorescent light, 16 hours daily
- Light intensity: approx. 1500 lux

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : oxygen, pH, temperature daily (in separate vessels at start of test)
Reference substance (positive control):
no
Key result
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
0.023 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: 0.017-0.031 mg/l; calculated
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
0.022 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: Graphically determined
Duration:
24 h
Dose descriptor:
EC0
Effect conc.:
0.001 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: in test
Duration:
24 h
Dose descriptor:
EC100
Effect conc.:
0.32 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: in test
Details on results:
Controls : Immobilization in blank : 0%
Validity criteria fulfilled:
not specified
Conclusions:
The effective concentration (EC50) value of test material in aquatic invertebrate (Daphnia Magna) in a 24 hr study on the basis of mobility was estimated to be 0.023 mg/l.
Executive summary:

Short term toxicity of test material was evaluated on aquatic invertebrate Daphnia magna for 24 hr according to EEC-Guideline L251 .Cultures of daphnia were maintained in glass vessels containing approx. 2.5 1 of reconstituted waterat 20 ± 1"C (water is renewed partially thrice weekly). At each renewal the daphnia are fed a suspension of green algae (Scenedesmus subspicatus) supplemented by a suspension of TETRAMIN-extract in such quantities that the food is consumed after 24h. In the pretest 24h before the start of the test reproductive daphnia were separated from the young by sieving all individuals through a 800 µm seive. This operation is repeated 6h before the start of the test and the young (6-24h of age) are retained for the test. Test was performed in beaker covered with watch glass having fill volume of 100 ml. The nominal concentrations at which test was performed were 0.0010, 0.0018, 0.0032, 0.0058, 0.010 0.018, 0.032, 0.058, 0.10, 0.18, 0.32 0.58, 1.0 mg/l. The EC50-values were calculated according to SPEARMAN-KAERBER in: D.J. Finney, 524-530, London (1964). EC-values were graphically determined on gausso-logarithmic probability paper. The effective concentration (EC50) value of test material in aquatic invertebrate (Daphnia Magna) in a 24 hr study on the basis of mobility was estimated to be 0.023 mg/l. Based on the above effect concentration it can be considered that the test material is highly toxic to aquatic invertebrates and can be classified as per CLP criteria.

Description of key information

Short term toxicity of test material was evaluated on aquatic invertebrate Daphnia magna for 24 hr according to EEC-Guideline L251 .Cultures of daphnia were maintained in glass vessels containing approx. 2.5 1 of reconstituted waterat 20 ± 1"C (water is renewed partially thrice weekly). At each renewal the daphnia are fed a suspension of green algae (Scenedesmus subspicatus) supplemented by a suspension of TETRAMIN-extract in such quantities that the food is consumed after 24h. In the pretest 24h before the start of the test reproductive daphnia were separated from the young by sieving all individuals through a 800 µm seive. This operation is repeated 6h before the start of the test and the young (6-24h of age) are retained for the test. Test was performed in beaker covered with watch glass having fill volume of 100 ml. The nominal concentrations at which test was performed were 0.0010, 0.0018, 0.0032, 0.0058, 0.010 0.018, 0.032, 0.058, 0.10, 0.18, 0.32 0.58, 1.0 mg/l. The EC50-values were calculated according to SPEARMAN-KAERBER in: D.J. Finney, 524-530, London (1964). EC-values were graphically determined on gausso-logarithmic probability paper. The effective concentration (EC50) value of test material in aquatic invertebrate (Daphnia Magna) in a 24 hr study on the basis of mobility was estimated to be 0.023 mg/l. Based on the above effect concentration it can be considered that the test material is highly toxic to aquatic invertebrates and can be classified as per CLP criteria.

Key value for chemical safety assessment

EC50/LC50 for freshwater invertebrates:
0.023 mg/L

Additional information

Short term toxicity of test material was evaluated on aquatic invertebrate Daphnia magna for 24 hr according to EEC-Guideline L251 .Cultures of daphnia were maintained in glass vessels containing approx. 2.5 1 of reconstituted waterat 20 ± 1"C (water is renewed partially thrice weekly). At each renewal the daphnia are fed a suspension of green algae (Scenedesmus subspicatus) supplemented by a suspension of TETRAMIN-extract in such quantities that the food is consumed after 24h. In the pretest 24h before the start of the test reproductive daphnia were separated from the young by sieving all individuals through a 800 µm seive. This operation is repeated 6h before the start of the test and the young (6-24h of age) are retained for the test. Test was performed in beaker covered with watch glass having fill volume of 100 ml. The nominal concentrations at which test was performed were 0.0010, 0.0018, 0.0032, 0.0058, 0.010 0.018, 0.032, 0.058, 0.10, 0.18, 0.32 0.58, 1.0 mg/l. The EC50-values were calculated according to SPEARMAN-KAERBER in: D.J. Finney, 524-530, London (1964). EC-values were graphically determined on gausso-logarithmic probability paper. The effective concentration (EC50) value of test material in aquatic invertebrate (Daphnia Magna) in a 24 hr study on the basis of mobility was estimated to be 0.023 mg/l. Based on the above effect concentration it can be considered that the test material is highly toxic to aquatic invertebrates and can be classified as per CLP criteria.