2-fluoro-6-trifluoromethylpyridine

Toxicity to soil macroorganisms except arthropods

Link to relevant study record(s)

Reference

Endpoint:

toxicity to soil macroorganisms except arthropods: short-term

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 207 (Earthworm, Acute Toxicity Tests)

Deviations:

no

Qualifier:

according to guideline

Guideline:

other: The Guidelines for the Testing of Chemicals, SEPA, R.R. China No. 207 "Earthworm, Acute Toxicity Test" (May 2004)

Deviations:

no

GLP compliance:

yes

Specific details on test material used for the study:

Substance ID: F6TF
Lot #: CZ1012090
Purity: 99.64%

Analytical monitoring:

not specified

Test organisms (species):

Eisenia fetida

Animal group:

annelids

Details on test organisms:

TEST ORGANISM
- Common name: Earthworm
- Source: Chenggong Earthworm Raising Farm, Dongli District, Tianjing
- Age at test initiation (mean and range, SD): adult (more than 2 months old with clitellum)
- Weight at test initiation (mean and range, SD): 300-527 mg

Study type:

laboratory study

Substrate type:

artificial soil

Limit test:

yes

Total exposure duration:

14 d

Test temperature:

18.17-19.66°C

pH:

Mean pH was 5.96.

Moisture:

Mean moisture content was 26.1%.

Details on test conditions:

TEST SYSTEM
- Test container (material, size): Labeled test container (height 20 cm; length 12 cm; width 10 cm)
- Amount of soil or substrate: ca. 777 g
- No. of organisms per container (treatment): 10
- No. of replicates per treatment group: 4
- No. of replicates per control: 4

SOURCE AND PROPERTIES OF SUBSTRATE (if soil): The artificial soil was prepared with 10% sphagnum peat moss, 20% kaolin clay, 68% quartz sand, and 2% calcium carbonate. 30 kg dry artificial soil was prepared just before the starting of the test. All the components were mixed with full stirring. Proper volume of distilled water was added to adjust soil moisture content. Moisture content was determined by sampling a small quantity of soil, dried at 105°C, and re-weighed.

OTHER TEST CONDITIONS
- Photoperiod: Light was kept continuous
- Light intensity: 435-453 Lux

EFFECT PARAMETERS MEASURED (with observation intervals if applicable): After 7 and 14 days, behavioural or pathological symptoms were recorded and the mortality in each container was assessed.

TEST CONCENTRATIONS: Based on the results of a preliminary toxicity test, the definitive study was conducted on a limit test with a concentration of 1000 mg/kg dry soil.

Nominal and measured concentrations:

1000 mg/kg dry soil (960.2 mg/kg dry soil as exposure)

Reference substance (positive control):

no

Key result

Duration:

14 d

Dose descriptor:

LC50

Effect conc.:

> 1 000 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Remarks on result:

other: 960.2 mg/kg dry soil as exposure

Details on results:

During the exposure period, no visible toxic signs or deaths were observed. Some earthworms in the test group became softer than the control, but the normal activity and escape capability had not been affected, and no mortality was observed. In the control group, no toxic signs or deaths were observed.

Validity criteria fulfilled:

yes

Conclusions:

14-Day LC50: >1000 mg/kg dry soil (960.2 mg/kg dry soil as exposure)

Executive summary:

The study was performed to assess the acute toxicity of the test substance to the earthworm (Eisenia foetide) according to the guideline OECD 207. Based on the result of a preliminary test, the definitive study was conducted as a limit test with a concentration of 1000 mg/kg_{dry soil} (960.2 mg/kg_{dry soil} as exposure). A concurrent control group was tested in parallel. For test and control groups, 4 replicates were used with 10 worms in each replicate. Artificial soil was used in this study. After 7 and 14 days, behavioural or pathological symptoms were recorded and the mortality in each container was assessed.

During the exposure period, no visible toxic signs or deaths were observed. Only some earthworms in the test group became softer than the control, but the normal activity and escape ability had not been affected, and no death was found. In the control group, no toxic signs or death were observed. According to the results, the 14-day LC₅₀ was >1000 mg/kg_{dry soil} (960.2 mg/kg_{dry soil} as exposure).

Description of key information

14-day LC50 >1000 mg/kg soil dry weight

Key value for chemical safety assessment

Short-term EC50 or LC50 for soil macroorganisms:

1 000 mg/kg soil dw