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EC number: 294-600-1 | CAS number: 91744-38-6
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
Description of key information
The acute toxicity of the test item to the freshwater invertebrate Daphnia magna has been investigated and gave a 48-Hour EL*50 value of greater than 100 mg/L loading rate WAF. The No Observed Effect Loading rate was 100 mg/L loading rate WAF.
This indicates that in the range of water solubility the test item was not toxic under the test conditions.
Key value for chemical safety assessment
Additional information
A study was performed to assess the acute toxicity of the test item to Daphnia magna. The method followed was designed to be compatible with the OECD Guidelines for Testing of Chemicals (April 2004) No 202, "Daphnia sp, Acute Immobilisation Test" referenced as Method C.2 of Commission Regulation (EC) No. 440/2008.
Due to the low aqueous solubility and complex nature of the test item for the purposes of the test the test item was prepared as a Water Accommodated Fraction (WAF). Following a preliminary range-finding test, twenty daphnids (4 replicates of 5 animals) were exposed to a Water Accommodated Fraction (WAF) of the test item at single nominal loading rate of 100 mg/L for 48 hours at a temperature of 21 °C to 22 °C under static test conditions. The number of immobilized Daphnia and any adverse reactions to exposure were recorded after 24 and 48 hours.
The 48-Hour EL*50 for the test item to Daphnia magna based on nominal loading rates was greater than 100 mg/L loading rate WAF. The No Observed Effect Loading rate was 100 mg/L loading rate WAF. This indicates that in the range of water solubility the test item was not toxic under the test conditions. It was considered unnecessary and unrealistic to test at loading rates in excess of 100 mg/L loading rate WAF. Given that a suitable method of chemical analysis could not be developed to measure the dissolved test item concentration present in the WAF it was considered appropriate to take samples for Total Organic Carbon (TOC) analysis as an alternative. Analysis of the 100 mg/L loading rate WAF test preparations at 0 and 48 hours showed measured test concentrations of 1.63 and 1.62 mg C/L respectively were obtained. Given that the toxicity cannot be attributed to a single component or a mixture of components, but to the test item as a whole the results were based on nominal loading rates only.
*EL = Effective Loading Rate
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