Trimethylenediamine

Endpoint summary

Additional information

Overall aquatic toxicity: Acutely harmful to aquatic organisms.

 

Short-term toxicity to fish:

With high probability acutely not harmful to fish.

Trimethylenediamine (CAS 109-76-2) was tested in a static acute toxicity test according to German Industrial Standard DIN 38412, part 15 with Leuciscus idus as test organism. The 96-h LC50 was greater than 100 mg/L (nominal; BASF AG 1984; study number 84/13). The test concentration were not analytically verified, but are assumed to be stable over the test period.

In a separate study by Brooke et al. (1984), the acute toxicity was tested with Pimephales promelas under flow-through conditions. The test procedure is similar to OECD 203. The test solutions were neutralised which resulted in a higher LC50. The 96-h LC50 was 1190 mg/L (measured).

 

Long-term toxicity to fish:

In Annex IX of Regulation (EC) No 1907/2006, it is laid down that long-term toxicity testing shall be proposed by the registrant if the chemical safety assessment indicates the need to investigate further the effects on aquatic organisms.

According to Annex I of this regulation, the chemical safety assessment triggers further action when the substance or the preparation meets the criteria for classification as dangerous according to Directive 67/548/EEC or Directive 1999/45/EC or is assessed to be a PBT or vPvB.

The hazard assessment of the trimethylenediamine reveals neither a need to classify the substance as dangerous for the environment, nor is it a PBT or vPvB substance.

Therefore, and for reasons of animal welfare, a long-term toxicity study in fish is not provided.

 

Short-term toxicity to aquatic invertebrates:

Acutely harmful to aquatic invertebrates.

The acute toxicity of trimethylenediamine (CAS 109-76-2) to aquatic invertebrates was tested according to Directive 79/831/EEC, Annex V, Part C.2. The test was performed with Daphnia magna under static conditions. The test concentrations were not analytically verified, but are assumed to be stable. The 48-h LC50 was determined to be 27 mg/L.

 

Long-term toxicity to aquatic invertebrates:

The chronic toxicity of trimethylenediamine (CAS 109-76-2) to aquatic invertebrates was tested in a 21-d reproduction test with Daphnia magna according to OECD 211; the test concentrations were analytically verified (BASF SE, 2014). Based on reproduction, the 21-d NOEC was determined to be ≥ 10 mg/L (nominal; analytically verified). The 21-d NOEC for aquatic invertebrates is used for the derivation of PNECs.

Toxicity to aquatic algae and plants:

With high probability acutely not harmful to aquatic algae.

The toxicity of trimethylenediamine (CAS 109-76-2) was tested in a growth inhibition test according to the German Industrial Standard DIN 38412, Part 9. The test species was Desmodesmus subspicatus. The test item was studied in two separate assays with either pH-adjusted or not pH-adjusted test solutions. The 72-h ErC50 was determined to be 175 mg/L if the test solutions were not pH-adjusted (nominal; BASF AG 1990; Study no. 2/0489/89). After pH adjustment no effects were noted, the 72-h NOErC was greater than 500 mg/L (nominal; BASF AG 1990; Study No. 2/w498/89). The test item concentrations were not analytically verified, but are assumed to be stable.

In a study conducted according to ISO 10253 (Water quality - Marine Algal Growth Inhibition Test with Skeletonema costatum and Phaeodactylum tricornutum) the growth rate of Skeletonema costatum was inhibited by 50% at a concentration of trimethylenediamine of 299 mg/l after 72 hours (Eide-Haugmo, 2009, 2012).

 

Toxicity to microorganisms:

The inhibition of the degradation activity of activated sludge is not anticipated when introduced in appropriately low concentrations.

The inhibiting effect of trimethylenediamine (CAS 109-76-2) on the respiration activity of domestic activated sludge was investigated in a study according to OECD 209. The 30 min EC20 was determined to be approximately 950 mg/L (BASF AG, 2002; Study No. 02/0218/08/1).

The results of a growth inhibition study with Pseudomonas putida according to German Industrial Standard DIN 38412, part 8 (BASF AG, 1989; Study No. 9/1890498) show a higher sensitivity than the OECD 209 study. The 17-h EC10 were determined to be 45 mg/L. According to the REACH Guidance R.10, test results according to Bringmann & Kühn (1980) which is equivalent to DIN 38412 , part 8 should only be used if no other test results are available. Therefore the short-term respiration study according to OECD 209 will be used for the derivation of the PNEC.