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EC number: 911-254-5 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- February 17 to April 22, 2010
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Remarks:
- Meets the requirements of GLP. There are no deviations from the recommended guideline that could affect the outcome of the study. One minor deviation is stated.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- yes
- Remarks:
- (the temperature was in a range of 22-25 ºC instead of 20 ± 2 ºC. This deviations was stated as uncritical, as normal respiration activity of the control could be observed).
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.11 (Biodegradation: Activated Sludge Respiration Inhibition Test)
- Deviations:
- yes
- Remarks:
- (the temperature was in a range of 22-25 ºC instead of 20 ± 2 ºC. This deviations was stated as uncritical, as normal respiration activity of the control could be observed).
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test item was added to the test vessel directly. - Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Pretreatment: sludge was washed with tap water three times, then centrifuged and re-suspended in tap water.
- Dry Matter:
Pre-treatment: the dry matter was determined as 3.08 g suspended solids/L, giving a concentration of 1.23 g suspended solids/L in the test.
Main test: the dry matter of the activated sludge was determined as 3.78 g suspended solids/L, giving a concentration of 1.51 g suspended solids/L in the test. - Test type:
- static
- Water media type:
- freshwater
- Total exposure duration:
- 3 h
- Test temperature:
- 22-25 ºC
- pH:
- Main test: 7.8-8.0
- Nominal and measured concentrations:
- Nominal concentrations: six concentrations ranging from 220 to 4.6 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: glass beakers
- Aeration: with purified air
- Type of flow-through (e.g. peristaltic or proportional diluter): approx. 0.75L/min.
- No. of vessels per concentration (replicates):
one replicate/treatment (positive control all experiments, test item treatment 10 and 1 mg/L pre-test)
two replicates/treatment (test item main study and treatment 1000 mg/L pre-test)
- No. of vessels per control (replicates):
two replicates before and two after measuring positive control and test item, respectively
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: tap water
- Total organic carbon: < 1.0 mg/L
- Pesticides: < LOD
- Conductivity: 142 µS/cm
TEST CONCENTRATIONS
- Test concentrations:
Pre-test: from 1000 to 1 mg/L (nominal concentration) (4 concentrations)
Main test: from 220 to 4.6 mg/L (nominal concentration) (6 concentrations) - Reference substance (positive control):
- yes
- Remarks:
- 3,5-Dichlorophenol (CAS 591-35-5)
- Key result
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 22 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Remarks on result:
- other: CI not determinable
- Duration:
- 3 h
- Dose descriptor:
- other: EC20
- Effect conc.:
- 41 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Remarks on result:
- other: 30 - 51 mg/L
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- 87 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Remarks on result:
- other: 71 - 110 mg/L
- Results with reference substance (positive control):
- - Results with reference substance valid? yes
- Relevant effect levels:
Study 3h-EC50 95% Confidence Interval
Pre-Test 14 mg/L not determinable
Main study 8.2 mg/L 4.2 - 12 mg/L - Validity criteria fulfilled:
- yes
- Conclusions:
- The following results for the test item were determined:
3h NOEC = 22 mg/L
3h EC20 = 41 mg/L (95% confid. interv.: 30 - 51 mg/l)
3h EC50 = 87 mg/L (95% confid. interv.: 71 - 110 mg/l) - Executive summary:
Two valid experiments were performed. In the pre-test, the test item was tested using four concentrations ranging from 1000 to 1 mg/L (nominal concentration). Duration of the test was three hours. Activated sludge was used as inoculum. It was taken from a domestic sewage treatment plant and washed before usage. The dry matter was determined as 3.08 g suspended solids/L, giving a concentration of 1.23 g suspended solids/L in the test. 3,5-Dichloro-phenole was used as positive control. Four concentrations were tested; an EC50 of 14 mg/L was determined, which lies within the demanded range of 5 – 30 mg/L. As inhibition was observed, a study plan amendment was issued and a main study was performed.
The main study was performed using six concentrations, ranging from 220 to 4.6 mg/L (nominal concentration). The dry matter of the activated sludge was determined as 3.78 g suspended solids/L, giving a concentration of 1.51 g suspended solids/L in the test. The EC50 of the positive control was determined as 8.2 mg/L, which lies within the demanded range of 5 – 30 mg/L. The following results for the test item were determined:
3h NOEC = 22 mg/L
3h EC20 = 41 mg/L (95% confid. interv.: 30 - 51 mg/l)3h EC50 = 87 mg/L (95% confid. interv.: 71 - 110 mg/l)
Reference
The test item showed inhibition of the respiration rate of activated sludge in concentrations above 22 mg/L. The highest treatment (220 mg/L) showed a mean inhibition value of 80 %. EC20 and EC50 were derived from the four treatments showing significant inhibition values and good correlation. The pre-test and the main studies show good correlation of the inhibitory effects of the test item. In the main study,O2 consumption in two controls (vessel no. 28 and 36) was considerably higher than in the other nine replicates. These replicate were stated as outliers and were not used for evaluation of the results. No observations were made which might cause doubts concerning the validity of the study outcome. The result of the test can be considered valid.
Description of key information
Key study: experimental result: test according to OECD guideline 209 ane EU method C.11. GLP study.
The 3-h EC50 for the test item was determined to be 87 mg/L.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 87 mg/L
- EC10 or NOEC for microorganisms:
- 22 mg/L
Additional information
Key study: experimental result: test according to OECD guideline 209 ane EU method C.11.
The main study was performed using six concentrations, ranging from 220 to 4.6 mg/L (nominal concentration).
The following results for the test item were determined:
Effect concentrations
Duration |
Endpoint |
Effect conc. |
Nominal/Measured |
Conc. based on |
Basis for effect |
Remarks (e.g. 95% CL) |
3 h |
NOEC |
22 mg/L |
test mat. |
respiration rate |
CI not determinable |
|
3 h |
other: EC20 |
41 mg/L |
test mat. |
respiration rate |
30 - 51 mg/L |
|
3 h |
EC50 |
87 mg/L |
test mat. |
respiration rate |
71 - 110 mg/L |
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