2,3-dichloroaniline

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

other information

Study period:

2022

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: No reliability is given as this is an applicant's summary entry for a variety of studies.

Data source

Reference

Reference 1

Reference Type:

publication

Title:

A comparative study of the toxicity of 1,1,2-trichloroethane, Dieldrin, Pentachlorophenol and 3,4-dichloroaniline for marine and fresh water organisms

Author:

Adema DMM, Vink GJ

Year:

1981

Bibliographic source:

Chemosphere Vol. 10, No. 6, 533-554

Reference 2

Reference Type:

other: secondary source

Title:

3,4-dichloroaniline (3,4-DCA)

Author:

EU-Risk Assessment Report

Year:

2006

Bibliographic source:

EUR 22235 EN; ISSN 1018-5593

Reference 3

Reference Type:

other: Expert Statement

Title:

2,3-dichloroaniline (CAS 608-27-5) Read-across justification

Year:

2022

Materials and methods

Principles of method if other than guideline:

TNO (1980): Degradability, Ecotoxicity and Bioaccumulation; The determination of the possible effects of chemicals and wastes on the aquatic environment. Government Publishing Office, The Hague, The Netherlands.

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

2,3-dichloroaniline

EC Number:

210-157-9

EC Name:

2,3-dichloroaniline

Cas Number:

608-27-5

Molecular formula:

C6H5Cl2N

IUPAC Name:

2,3-dichloroaniline

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

Sampling method: concentration of the test compound was measured by chemical analysis during the test and was at least 70% of the nominal value

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Age at study initiation: life stage: larvae
- Length at study initiation: 1 mm

Study design

Test type:

not specified

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

pH:

the pH was measured during the test and was always about 8

Dissolved oxygen:

the oxygen concentration was measured during the test and was never less than 70% of the saturation value

Nominal and measured concentrations:

measured concentration was at least 70% of the nominal value

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

0.16 mg/L

Conc. based on:

test mat.

Basis for effect:

mortality

Effect concentrations 2

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

0.23 mg/L

Conc. based on:

test mat.

Basis for effect:

mortality

Any other information on results incl. tables

Read across (3,4-dichloroaniline): LC50 (Daphnia, 48-h): 0.23 mg/L (Adema. and Vink, 1981; cited in EU RAR, 2006) 

Applicant's summary and conclusion

Conclusions:

The most critical valid short-term LC50 (48h) is 0.23 mg/L on Daphnia magna (Adema. and Vink, 1981) which was experimentally determined.

Executive summary:

In this read-across approach data on 3,4-dichloroaniline (CAS 95-76-1, source substance) is used to fill data gaps for 2,3-dichloroaniline (CAS 608-27-5, target substance). According to the “Read-Across Assessment Framework” (RAAF) (ECHA 2017) the analogue approach is followed. Data on 3,4-dichloroaniline (CAS 95-76-1) is used to fill data gaps for 2,3-dichloroaniline (CAS 608-27-5). The target substance is a basically an isomer of the source substance. The physical and chemical properties of the source and the target substance are in the same range. Both, the source and the target substance have a log Pow value of less than 3, which implies a low potential to bioaccumulation. Thus, the source and the target substance may be excreted quickly and are not expected to bioaccumulate in the food chain. However, 3,4-dichloroaniline has shown significant ecotoxicity based on tests done on fish, daphnids and algae. Hence, based on the structural similarity, the similar physico-chemical properties, and the fact that the structural difference in the source substance indicates a slightly higher toxicity when compared to the target substance, the target substance as such does not need to be tested in aqueous system. It is sufficient for hazard assessment to use the data gained with 3,4-dichloroaniline. This can be regarded as worst-case for the target substance. For more details please see attached read-across statement.