3,7-dimethylnona-2,6-dienenitrile

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

weight of evidence

Study period:

No date specified

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

other: Documentation insufficient for assessment

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1969

Report date:

1969

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

Oral intubation of 5 groups consisting of six rats (males) each. Bodyweights of test animals ranged from 148 to 200 grams. the animals were fasted 12 hours prior to dosing. Observations for mortality and signs of effect were made for 7 days and survivors were sacrificed. LD50 was calculated by method of Miller and Tainter.

GLP compliance:

no

Remarks:

Study predates GLP

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

(2Z/6Z)-3,7-dimethylnona-2,6-dienenitrile

Molecular formula:

C11H17N

IUPAC Name:

(2Z/6Z)-3,7-dimethylnona-2,6-dienenitrile

Constituent 2

Reference substance name:

(2Z/6E)-3,7-dimethylnona-2,6-dienenitrile

Molecular formula:

C11H17N

IUPAC Name:

(2Z/6E)-3,7-dimethylnona-2,6-dienenitrile

Constituent 3

Reference substance name:

(2E/6Z)-3,7-dimethylnona-2,6-dienenitrile

Molecular formula:

C11H17N

IUPAC Name:

(2E/6Z)-3,7-dimethylnona-2,6-dienenitrile

Constituent 4

Reference substance name:

(2E/6E)-3,7-dimethylnona-2,6-dienenitrile

Molecular formula:

C11H17N

IUPAC Name:

(2E/6E)-3,7-dimethylnona-2,6-dienenitrile

impurity 1

Reference substance name:

(3E/6Z)-3,7-dimethylnona-3,6-dienenitrile

Molecular formula:

C11H17N

IUPAC Name:

(3E/6Z)-3,7-dimethylnona-3,6-dienenitrile

impurity 2

Reference substance name:

(3E/6E)-3,7-dimethylnona-3,6-dienenitrile

Molecular formula:

C11H17N

IUPAC Name:

(3E/6E)-3,7-dimethylnona-3,6-dienenitrile

Test material form:

liquid

Specific details on test material used for the study:

- Name of test material (as cited in study report): Lemonile; GIV 2-1334
- Appearance: Clear liquid, lemon odour

Test animals

Species:

rat

Strain:

not specified

Sex:

male

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Weight at study initiation: 148 - 200 g
- Fasting period before study: 12 hours

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

unchanged (no vehicle)

Doses:

100, 316, 1000, 2000 and 4000 mg/kg bw

No. of animals per sex per dose:

6 males/dose

Control animals:

no

Details on study design:

Observations for mortality and signs of effect were made for 7 days and survivors were sacrificed.

Statistics:

The LD50 was calculated by the method of Miller and Tainter

Results and discussion

Effect levels

Sex:

male

Dose descriptor:

LD50

Effect level:

2 000 mg/kg bw

Based on:

test mat.

95% CL:

383

Mortality:

See Table 1

Clinical signs:

other: Principle toxic effects were depression, flushing of extremities and tremors which were seen at the high dose level.

Any other information on results incl. tables

Table 1: Mortality

Dose (mg/kg)	% cumulative mortality
100	0.0
316	0.0
1000	16.6
2000	50.0
4000	100.0

 

Applicant's summary and conclusion

Interpretation of results:

GHS criteria not met

Conclusions:

The acute oral median lethal dose (LD50) of the test material in male rats was 2000 mg/kg bodyweight.