2,2'-[(3,3'-dichloro[1,1'-biphenyl]-4,4'-diyl)bis(azo)]bis[N-(4-ethoxyphenyl)-3-oxobutyramide]

Bioaccumulation: aquatic / sediment

Administrative data

Endpoint:

bioaccumulation in aquatic species: fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1999-12-17 - 2000-03-02

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2000

Report date:

2000

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 305 (Bioconcentration: Flow-through Fish Test)

Deviations:

yes

Remarks:

Only one test substance concentration (at least two concentrations recommended by OECD 305)

Test guideline 2

Qualifier:

according to guideline

Guideline:

other: "Method for Testing the Degree of Accumulation of Chemical Substances in Fish Body" stipulated in the "Testing Methods for New Chemical Substances" (July 13, 1974, Revised October 8, 1998, Kanpogyo No.5, Environment Agency, Japan)

GLP compliance:

yes

Remarks:

"OECD Principles of Good Laboratory Practice" (May 12, 1981)

Test material

Test material information

Constituent 1

Reference substance name:

2,2'-[(3,3'-dichloro[1,1'-biphenyl]-4,4'-diyl)bis(azo)]bis[N-(2,4-dimethylphenyl)-3-oxobutyramide]

EC Number:

225-822-9

EC Name:

2,2'-[(3,3'-dichloro[1,1'-biphenyl]-4,4'-diyl)bis(azo)]bis[N-(2,4-dimethylphenyl)-3-oxobutyramide]

Cas Number:

5102-83-0

IUPAC Name:

2,2'-[(3,3'-dichlorobiphenyl-4,4'-diyl)didiazene-2,1-diyl]bis[N-(2,4-dimethylphenyl)-3-oxobutanamide]

Radiolabelling:

no

Test solutions

Vehicle:

yes

Test organisms

Test organisms (species):

Cyprinus carpio

Study design

Route of exposure:

aqueous

Test type:

flow-through

Water / sediment media type:

natural water: freshwater

Total exposure / uptake duration:

28 d

Total depuration duration:

0 d

Test conditions

Hardness:

Total hardness (Ca, Mg): 132 mg/L

Test temperature:

Test group: 24.8 - 25.8 °C
Control gorup: 25.3 - 25.8 °C

pH:

7.6

Dissolved oxygen:

Ca. 8 mg/L, continuously monitored during the study.

TOC:

1.3 mg/L

Nominal and measured concentrations:

Nominal: 0,1 mg/L
Mean measured: 0.0935 mg/L

Reference substance (positive control):

no

Results and discussion

Lipid content

Lipid content 1

Lipid content:

1.69 %

Time point:

start of exposure

Lipid content 2

Lipid content:

1.96 %

Time point:

end of exposure

Bioaccumulation factor

Type:

BCF

Value:

>= 0 - <= 6.2 dimensionless

Basis:

whole body w.w.

Calculation basis:

steady state

Remarks:

As no time of plateau could be determined, days of analysis 14, 21 and 28 were taken for the calculation of the steady state concentration in the test medium and the relation to the limit of determination in fish gave the upper limit of BCF of 6.2.

Remarks on result:

other: The concentration in none of the 5 treatment samples analyzed during the 28 d period was above the limit of determination. As such, no time of plateau could be determined.

Remarks:

Conc.in environment / dose:0.0935 mg/L

Depuration

Elimination:

not specified

Parameter:

other: The concentration in none of the 5 treatment samples analyzed during the 28 d period was above the limit of determination. As such, no analysis of depuration was possible.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes