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Ecotoxicological information

Toxicity to microorganisms

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Reference
Endpoint:
toxicity to microorganisms, other
Type of information:
read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
data from handbook or collection of data
Remarks:
Experimental data of various test chemicals
Justification for type of information:
Data for the target chemical is from collection of data
Reason / purpose for cross-reference:
read-across source
Reason / purpose for cross-reference:
read-across source
Qualifier:
according to guideline
Guideline:
other: Refer below principle
Principles of method if other than guideline:
WoE report is based on toxicity study of microorganism for the test chemical:
WoE 2 and 3rd
GLP compliance:
no
Analytical monitoring:
no
Vehicle:
no
Test organisms (species):
other: S. aureus, B. cereus , E. coli , P. aeruginosa
Test type:
static
Water media type:
freshwater
Total exposure duration:
48 h
Details on test conditions:
2nd and 3rd study:

Test vessel: Petri plate
-The turbidity of microorganisms was adjusted to 0.5 McFarland. The inoculum were 1×108 1×106 CFU/ml for bacteria and fungi respectively. Inoculate was wabbed on Muller Hinton Agar.Sterile blank discs impregnated with 3, 5, 10 μl of oil in 10 μl of dimethyl sulfoxide (DMSO) were used and put on cultured plates.
Reference substance (positive control):
no
Key result
Duration:
48 h
Dose descriptor:
other: MIC
Effect conc.:
> 8 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth inhibition
Remarks on result:
other: 2nd study
Duration:
48 h
Dose descriptor:
other: MIC
Effect conc.:
1 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth inhibition
Remarks on result:
other: 3rd study
Validity criteria fulfilled:
no
Conclusions:
The test chemical is likely to be toxic to microorganism atleast in the concentartion range of 1 to 8 mg/l.
Executive summary:

Data available for the test chemicals has been reviewed to determine its effect on microoranism.The studies are as mentioned below:

In the first study toxicity of test material was evaluated for microorganisms by disc diffusion method and the minimum inhibition concentration was evaluated using broth dilution method. The organisms used for the test were S. aureus, B. cereus , E. coli , P. aeruginosa. The turbidity of microorganisms was adjusted to 0.5 McFarland. The inoculum were 1×1081×106CFU/ml for bacteria and fungi respectively. Inoculate was swabbed on Muller Hinton Agar. Sterile blank discs impregnated with 3, 5, 10 μl of oil in 10 μl of dimethyl sulfoxide (DMSO) were used and put on cultured plates. Disc containing DMSO and antibiotics were used as control. The minimum inhibition concentration of test material on bacteria S. aureus, B. cereus , E. coli , P. aeruginosa was observed to be > 8 mg/l.

Above study was supported by the second from secondary source. Toxicity of test material was evaluated for microorganisms by disc diffusion method and the minimum inhibition concentration was evaluated using broth dilution method. The organisms used for the test were S. aureus, B. cereus , E. coli , P. aeruginosa. The turbidity of microorganisms was adjusted to 0.5 McFarland. The inoculum were 1×1081×106CFU/ml for bacteria and fungi respectively. Inoculate was swabbed on Muller Hinton Agar. Sterile blank discs impregnated with 3, 5, 10 μl of oil in 10 μl of dimethyl sulfoxide (DMSO) were used and put on cultured plates. Disc containing DMSO and antibiotics were used as control. The minimum inhibition concentration of test material on bacteria S. aureus, B. cereus , E. coli , P. aeruginosa was observed to be 1 mg/l.

The test chemical is likely to be toxic to microorganism  atleast in the concentartion range of 1 to 8 mg/l.

Description of key information

The test chemical is likely to be toxic to microorganism  atleast in the concentartion range of 1 to 8 mg/l.

Key value for chemical safety assessment

EC50 for microorganisms:
1 mg/L

Additional information

Data available for the test chemicals has been reviewed to determine its effect on microoranism.The studies are as mentioned below:

In the first study toxicity of test material was evaluated for microorganisms by disc diffusion method and the minimum inhibition concentration was evaluated using broth dilution method. The organisms used for the test were S. aureus, B. cereus , E. coli , P. aeruginosa. The turbidity of microorganisms was adjusted to 0.5. The inoculum were 1×1081×106CFU/ml for bacteria and fungi respectively. Inoculate was swabbed on Muller Hinton Agar. Sterile blank discs impregnated with 3, 5, 10 μl of oil in 10 μl of dimethyl sulfoxide (DMSO) were used and put on cultured plates. Disc containing DMSO and antibiotics were used as control. The minimum inhibition concentration of test material on bacteria S. aureus, B. cereus , E. coli , P. aeruginosa was observed to be > 8 mg/l.

Above study was supported by the second from secondary source. Toxicity of test material was evaluated for microorganisms by disc diffusion method and the minimum inhibition concentration was evaluated using broth dilution method. The organisms used for the test were S. aureus, B. cereus , E. coli , P. aeruginosa. The turbidity of microorganisms was adjusted to 0.5. The inoculum were 1×1081×106CFU/ml for bacteria and fungi respectively. Inoculate was swabbed on Muller Hinton Agar. Sterile blank discs impregnated with 3, 5, 10 μl of oil in 10 μl of dimethyl sulfoxide (DMSO) were used and put on cultured plates. Disc containing DMSO and antibiotics were used as control. The minimum inhibition concentration of test material on bacteria S. aureus, B. cereus , E. coli , P. aeruginosa was observed to be 1 mg/l.

The test chemical is likely to be toxic to microorganism  atleast in the concentartion range of 1 to 8 mg/l.

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