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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: non-GLP Screening Test, however carried out at a GLP lab following OECD testing guidelines.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2009
Report date:
2009

Materials and methods

Principles of method if other than guideline:
Screening study similar to OECD 202 guideline.
GLP compliance:
yes
Remarks:
non-GLP screening test following OECD testing guidelines

Test material

Constituent 1
Chemical structure
Reference substance name:
Reaction mass of ethyl (2S,3S)-3,4-dibromo-2-methylbutanoate and ethyl (2R,3R)-3,4-dibromo-2-methylbutanoate and ethyl (2S,3R)-3,4-dibromo-2-methylbutanoate and ethyl (2R,3S)-3,4-dibromo-2-methylbutanoate
EC Number:
700-225-0
Cas Number:
1160806-44-9
Molecular formula:
C7 H12 Br2 O2
IUPAC Name:
Reaction mass of ethyl (2S,3S)-3,4-dibromo-2-methylbutanoate and ethyl (2R,3R)-3,4-dibromo-2-methylbutanoate and ethyl (2S,3R)-3,4-dibromo-2-methylbutanoate and ethyl (2R,3S)-3,4-dibromo-2-methylbutanoate
Details on test material:
- Name of test material (as cited in study report): 2-Methyl-3,4-dibrombuttersaeureethylester
- Analytical purity: 98.1 w-%
- Lot/batch No.: 35684-12-5

Test solutions

Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test substance was weighed separately for each concentration (differential loading) and stirred in M4 medium for approx. 1 hour at 20 °C. The stock solutions 1 and 10 mg/L were clear and colorless. The stock solution 100 mg/L was clear, oil drops were visible on the bottom of the test vessel.

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Source: The clone of Daphnia magna was bred at the testing facility.
- Age at study initiation (mean and range, SD): <24 h
- Feeding during test: none

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h

Test conditions

Nominal and measured concentrations:
Nominal test concentrations: 0, 1, 10, 100 mg/L
Details on test conditions:
TEST SYSTEM
- Fill volume: 10 ml
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4



TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: according to OECD guideline 202
- Total organic carbon:
- Particulate matter:
- Metals:
- Pesticides:
- Chlorine:
- Alkalinity:
- Ca/mg ratio:
- Conductivity:
- Culture medium different from test medium:
- Intervals of water quality measurement:


OTHER TEST CONDITIONS
- Adjustment of pH:
- Photoperiod:
- Light intensity:


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Mobility after 24 and 48 h

TEST CONCENTRATIONS
- Spacing factor for test concentrations:
- Justification for using less concentrations than requested by guideline:
- Range finding study
- Test concentrations:
- Results used to determine the conditions for the definitive study:

Results and discussion

Effect concentrationsopen allclose all
Duration:
48 h
Dose descriptor:
EC0
Effect conc.:
10 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
10 - 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC100
Effect conc.:
100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
The test batches were elear solutions over the exposure period according to visual inspection.

Applicant's summary and conclusion