Potassium hydrogen tartrate

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

weight of evidence

Justification for type of information:

Endpoint-specific justification for readacross

Reason / purpose for cross-reference:

read-across source

Key result

Parameter:

other: BOD (%)

Remarks:

Indirect Analysis

Value:

ca. 75

Sampling time:

2 wk

Key result

Parameter:

% degradation (TOC removal)

Remarks:

Direct Analysis

Value:

ca. 92

Sampling time:

2 wk

Key result

Parameter:

other: HPLC (%)

Value:

ca. 100

Sampling time:

2 wk

.

Conclusions:

On average, 75% biodegradation of the analogue substance disodium tartrate is reported in a Japanese MITI sponsored GLP compliant study after 14 d. Result expressed in terms of BOD. Degradation of 92% and 100% obtained if monitored parameter is remaining TOC or test substance.

Executive summary:

On average, 75% biodegradation of the analogue substance disodium tartrate is reported in a Japanese MITI sponsored GLP compliant study after 14 d. Result expressed in terms of BOD. Degradation of 92% and 100% obtained if monitored parameter is remaining TOC or test substance.

Reference 2

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

weight of evidence

Justification for type of information:

Endpoint-specific justification for readacross

Reason / purpose for cross-reference:

read-across source

Key result

Parameter:

other: BOD (%)

Remarks:

Indirect Analysis

Value:

ca. 76

Sampling time:

2 wk

Key result

Parameter:

% degradation (TOC removal)

Value:

ca. 100

Sampling time:

2 wk

Key result

Parameter:

other: HPLC (%)

Value:

ca. 100

Sampling time:

2 wk

Conclusions:

On average, 76% biodegradation of the analogue substance tartaric acid is reported in a Japanese MITI sponsored GLP compliant study after 14 d in terms of BOD, and where 100% degradation is obtained when parameter chosen is TOC or test substance removal.

Executive summary:

On average, 76% biodegradation of the analogue substance tartaric acid is reported in a Japanese MITI sponsored GLP compliant study after 14 d in terms of BOD, and where 100% degradation is obtained when parameter chosen is TOC or test substance removal.

Reference 3

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

weight of evidence

Study period:

2001

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: The Official Bulletin of the Ministry of International Trade and Industry, Japan

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I))

Deviations:

no

GLP compliance:

yes

Specific details on test material used for the study:

Anhydrous disodium tartate used as test substance (CAS: 868-18-8). Referred to as K-1129 in the report, with a mol wt of 194.05 and a reported purity of 99.8%. Batch tested reported as M3R8322. Original report available only in Japanese.

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic (adaptation not specified)

Details on inoculum:

Concentration of activated sludge: 30 mg/L Cultivation conditions reported as regular. Aerobic conditions maintaine in a closed system.
Innoculum obtained from 10 samples obtained from waste water treatment plants in: Fushiko River plant (Sapporo-shi, Hokkaido), Nakahama plant (Osaka-shi, Osaka), Kitakami River (Ishinomaki-shi, Miyagi), Yoshinogawa (Tokushima-shi, Tokushima), Gulf of Hiroshima (Hiroshima-shi, Hiroshima), Fukashiba plant (Fashima-gun, Ibaraki), Ochiai plant (Shinjuku-ku, Tokyo), The Shinano River (Nishikanbara-gun, Niigata), Lake Biwa-ko (Ootsu-shi, Shiga), Gulf of sinus Sea (Kitakyuushuu-shi, Fukuoka). Inoculum mixed and prefiltered. Glucose / peptone medium used for cultivation. Dissoolved oxygen, pH and temperature of sludge culture is measured during culture and subjected to microscoic examination for abnormalities. None were detected.

Duration of test (contact time):

ca. 14 d

Initial conc.:

ca. 100 mg/L

Based on:

test mat.

Remarks:

Chemical Concentration

Parameter followed for biodegradation estimation:

O2 consumption

Parameter followed for biodegradation estimation:

TOC removal

Parameter followed for biodegradation estimation:

test mat. analysis

Remarks:

HPLC

Details on study design:

Test substance concentration = 100 mg/L. Test performed in triplicate test vessels at a temperature of 25 ±1 C°.
Water + test substance as well as a control blank were included in the experimental design. Test substance reported as stable under storage conditions.

Reference substance:

aniline

Remarks:

aniline (Batch number 2823. Reagent grade. Manufactured by Showa chemicals.)

Test performance:

Degradation of reference substance, expressed in terms of BOD was 63% at day 7 and 73% at day 14. Therefore test conditions are considered acceptable.

Key result

Parameter:

% degradation (O2 consumption)

Value:

ca. 75

St. dev.:

4.73

Sampling time:

14 d

Key result

Parameter:

% degradation (TOC removal)

Value:

ca. 92

St. dev.:

1

Sampling time:

14 d

Key result

Parameter:

% degradation (test mat. analysis)

Value:

ca. 100

St. dev.:

0

Sampling time:

14 d

Results with reference substance:

Degradation of reference substance, expressed in terms of BOD was 63% at day 7 and 73% at day 14. Therefore test conditions are considered acceptable.

.

Validity criteria fulfilled:

yes

Remarks:

(Degradation of reference substance, expressed in terms of BOD was 63% at day 7 and 73% at day 14. Therefore test conditions are considered acceptable.)

Interpretation of results:

readily biodegradable

Conclusions:

Biodegradation at 14 d expressed in terms of BOD with respecto to ThOD is >60%. Thereby according to criteria for OECD method 301C, the substance is considered to be readily biodegradable.

Indirect Analysis --> BOD = 75%

Direct Analysis --> TOC = 92%; HPLC = 100%

Executive summary:

Report on test performed in 1994 under Chemicals Programme sponsored by Japanese Ministry of International Trade and Industry (MITI) according to OECD Guideline 301C and under GLP indicates that analogue sodium ditartrate is readily biodegradable under specified test conditions after 14d. Biodegradability was estimated according to three parameters: BOD reduction, TOC reduction and test substance reduction as estimated by HPLC. The minimum average % biodegradation (mean of three replicates) obtained was of 75% in terms of BOD and of 100% as determined by substance disappearance estimated by HPLC analysis. Thereby, as predicted from its molecular structure disodium tartrate is considered to be readily biodegradable, as > 60% biodegradation is obtained in the MITI test. This analogue is considered to be a suitable predictor of the biodegradability of Potassium Sodium Tartrate, as the organic group of the submission substance is identical, being the only difference the exchange of one sodium atom for a potassium atom, which is irrelevant in terms of the degradable organic portion of the molecule.

Reference 4

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

weight of evidence

Study period:

2001

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: he Official Bulletin of the Ministry of International Trade and Industry, Japan

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I))

GLP compliance:

yes

Specific details on test material used for the study:

Anhydrous DL-tartaric acid is used as test substance. Referred to as K-1128 in the report, with a mol wt of 150.09 and a reported purity of > 99%. Batch tested reported as AL01. Original report available only in Japanese.

Oxygen conditions:

aerobic

Inoculum or test system:

mixture of sewage, soil and natural water

Details on inoculum:

Concentration of activated sludge: 30 mg/L Cultivation conditions reported as regular. Aerobic conditions maintained in a closed system.
Innoculum obtained from municipal wastewater sewage plant plus water and soil from Namiuti lake. Sludge culture density was determined to be 5400 mg/L. Final concentration in 300 ml test vessels = 30 mg/L.
Inoculum mixed and prefiltered. Glucose / peptone medium used for cultivation. Dissoolved oxygen, pH and temperature of sludge culture is measured during culture and subjected to microscoic examination for abnormalities. None were detected.

Duration of test (contact time):

ca. 14 d

Initial conc.:

ca. 100 mg/L

Based on:

test mat.

Remarks:

Chemical Concentration

Parameter followed for biodegradation estimation:

O2 consumption

Parameter followed for biodegradation estimation:

TOC removal

Parameter followed for biodegradation estimation:

test mat. analysis

Remarks:

HPLC

Details on study design:

Test substance concentration = 100 mg/L. Test performed in triplicate 300 ml test vessels at a temperature of 25 ±1 C°. Sludge density in test is reporte d to be 30 mg/L. Water + test substance as well as a control blank were included in the experimental design. Test substance reported as stable under storage conditions.

Reference substance:

aniline

Remarks:

aniline (Batch number SE-2823. Reagent grade. Manufactured by Showa chemicals.)

Test performance:

Degradation of reference substance, expressed in terms of BOD was 66% after 7 days and of 71% at day 14. Therefore test is considered acceptable.

Key result

Parameter:

% degradation (O2 consumption)

Remarks:

Indirect Analysis

Value:

ca. 76

St. dev.:

1.3

Sampling time:

14 d

Key result

Parameter:

% degradation (TOC removal)

Value:

ca. 100

St. dev.:

0

Sampling time:

14 d

Key result

Parameter:

% degradation (test mat. analysis)

Value:

ca. 100

St. dev.:

0

Sampling time:

14 d

Details on results:

A TOC-5000 analyser manufactured by Shimazu Corporation weas used. 0.9 mg C/ L estimated detection limit. TC furnace 680 °C. Flow rate 150 ml/min. Inject ion volume 33 μl. Test substance analysed using HPLC (LC-10AD Pons Shimazu, detector: SPD-10AV). Coloumn - TSKgel OAPAK-A 80 cmx 7. 8mm diameter. Elution 0.9 mmol/L; Flow: 1.0 ml/min; wawelength: 210 nm; injection volume: 20 μL; recorder range: 10 mV.

Results with reference substance:

Degradation of reference substance, expressed in terms of BOD was 63% at day 7 and 73% at day 14. Therefore test conditions are considered acceptable.

Validity criteria fulfilled:

yes

Remarks:

(Degradation of reference substance, expressed in terms of BOD was 63% at day 7 and 73% at day 14. Therefore test conditions are considered acceptable.)

Interpretation of results:

readily biodegradable

Conclusions:

Biodegradation at 14 d expressed in terms of BOD with respect to ThOD is >60%. Thereby according to criteria for OECD method 301C, the substance is considered to be readily biodegradable.

Indirect Analysis --> BOD = 76%

Direct Analysis --> TOC = 100%; HPLC = 100%

Executive summary:

Report on test performed in 1994 under Chemicals Programme sponsored by Japanese Ministry of International Trade and Industry (MITI) according to OECD Guideline 301C and under GLP indicates that DL-tartaric acid is readily biodegradable under specified test conditions after 14d. Biodegradability was estimated according to three parameters: BOD reduction, TOC reduction and test substance reduction as estimated by HPLC. The minimum average % biodegradation (mean of three replicates) obtained was of 76% in terms of BOD and the maximum was 100% as determined by substance disappearance estimated by HPLC analysis and by TOC analysis. Thereby, as predicted from its molecular structure tartaric acid is considered to be readily biodegradable, as > 60% biodegradation is obtained in the MITI test. This analogue is considered to be a suitable predictor of the biodegradability of Potassium Sodium Tartrate, as the organic group of the submission substance is identical.

Description of key information

On average, 75% biodegradation of the analogue substance disodium tartrate is reported in a Japanese MITI sponsored GLP compliant study after 14 d. Result expressed in terms of BOD. Degradation of 92% and 100% obtained if monitored parameter is remaining TOC or test substance. Comparable results are obtained with tartaric acid in an identical study performed by MITI, where 76% degradation is obtained after 14 d, in terms of BOD, and where 100% degradation is obtained when parameter chosen is TOC or test substance removal.

Key value for chemical safety assessment

Biodegradation in water:

readily biodegradable

Type of water:

freshwater

Additional information

Report on test performed in 1994 under Chemicals Programme sponsored by Japanese Ministry of International Trade and Industry (MITI) according to OECD Guideline 301C and under GLP indicates that analogue sodium ditartrate is readily biodegradable under specified test conditions after 14d. Biodegradability was estimated according to three parameters: BOD reduction, TOC reduction and test substance reduction as estimated by HPLC. The minimum average % biodegradation (mean of three replicates) obtained was of 75% in terms of BOD and of 100% as determined by substance disappearance estimated by HPLC analysis. Thereby, as predicted from its molecular structure disodium tartrate is considered to be readily biodegradable, as > 60% biodegradation is obtained in the MITI test. This analogue is considered to be a suitable predictor of the biodegradability of Potassium Sodium Tartrate, as the organic group of the submission substance is identical, being the only difference the exchange of one sodium atom for a potassium atom, which is irrelevant in terms of the degradable organic portion of the molecule. Comparable results are obtained when the substance tested in the source acid, DL-tartaric acid.