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EC number: 213-590-1 | CAS number: 991-84-4
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1985-03-13 to 1985-04-10
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Non-GLP study. Guideline from 1981. Deviation to the updated OECD Guideline (adopted 1992): The volume of the test solution was reduced fron 3.0 to 1.5 L.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- Deviations:
- yes
- Remarks:
- : volume of test solution reduced to 1.5 L
- GLP compliance:
- no
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge: Bacteria collected from activated sludge of the sewage treatment plant of CH - 4153 Reinach on 12/03/85
- Preparation of inoculum for exposure: according to guideline - Duration of test (contact time):
- 28 d
- Initial conc.:
- 10 mg/L
- Based on:
- test mat.
- Initial conc.:
- 20 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- TEST CONDITIONS
- Solubilising agent: in 300 mL of mineral solution were added 0.5 mL Nonylphenol 10E05P0 (solution of 31.2 mg in 100 ml bidist. water)
- Test temperature: 22 ± 2°C
TEST SYSTEM
- Culturing apparatus: 2 Liter flasks equipped with gas inlet and magnetic stirrer.
- Number of culture flasks/concentration: 1
- Test performed in closed vessels
- Method used to create aerobic conditions: 50 ml/min aeration free of carbon dioxide
SAMPLING
- Sampling frequency: day 5 / 9 / 13 / 16 / 19 / 22 / 27 and 28
- Sampling method: Titration of the carbondioxide, absorbed in the absorbers filled with 0.025 N Barium hydroxide on the days 5 / 9 / 13 / 16 / 19 / 22 / 27 and 28. - Reference substance:
- aniline
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 5
- Sampling time:
- 28 d
- Remarks on result:
- other: 10 mg test substance/L
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 7
- Sampling time:
- 28 d
- Remarks on result:
- other: 20 mg test substance/L
- Details on results:
- Theoretical carbon dioxide formation:
10 mg test substance/L 37.00 mg CO2/1.5 L
20 mg test substance/L 74.01 mg CO2/1.5 L - Results with reference substance:
- The biodegradation was calculated as 20 mg/L 93 % in 28 days.
- Interpretation of results:
- under test conditions no biodegradation observed
- Endpoint:
- biodegradation in water: screening tests
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- 1985-03-14
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Non GLP. Deivations: The test substance was dissolved in sulfuric acid containing silver sulfate instead of water
- Qualifier:
- according to guideline
- Guideline:
- other: DIN 38 409
- Deviations:
- yes
- GLP compliance:
- no
- Oxygen conditions:
- aerobic
- Duration of test (contact time):
- 2 h
- Details on study design:
- TEST CONDITIONS
- Composition of medium:
Potassium dichromate
Mercury sulfate
Silver sulfate
Sulfuric acid
- Test temperature: 148 +/- 3 °C
- Other:
Titration solution: Ammonia-iron-II-sulfate 0.012 mol/L. The preparation of the stock solution of the reagents was carried out according the method described in the guideline DIN 3O409
TEST SYSTEM
- Culturing apparatus: 250 mL round bottom flasks equiped with a condenser. - Reference substance:
- other: Potassium hydrogen phthalate
- Parameter:
- COD
- Value:
- 2.41 other: g COD/g test item
- Results with reference substance:
- 201 mg COD/L
Referenceopen allclose all
Description of key information
Not readily biodegradable (according to OECD criteria); poorly biodegradable.
Key value for chemical safety assessment
Additional information
The ready biodegradability of the test item was assessed over a 28 day period by the modified Sturm test (OECD 301B). The nominal test concentrations were 10 and 20 mg a.s./L. The test material attained up to 5 and 7 % biodegradation after 28 days whereas the positive control substance (Aniline) was 93 % degraded after 28 days. These results indicate that the test item cannot be classified readily biodegradable under conditions of the test.
The COD calculted in a study according to DIN 38409 was determined to be 2.41 g COD/ g test item.
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