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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
toxicity to microorganisms
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1988
Report date:
1988

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: DIN 38412, part 8
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Methylurea
EC Number:
209-935-0
EC Name:
Methylurea
Cas Number:
598-50-5
Molecular formula:
C2H6N2O
IUPAC Name:
methylurea
Details on test material:
- Name of test material (as cited in study report): Methylharnstoff technisch

Sampling and analysis

Analytical monitoring:
no

Test organisms

Test organisms (species):
Pseudomonas putida

Study design

Test type:
static
Water media type:
freshwater
Total exposure duration:
17 h

Test conditions

Test temperature:
22 ± 1 °C
pH:
4.8 - 7.3
Nominal and measured concentrations:
Nominal test substance concentrations:
156.25, 312.5, 625, 1250, 2500, 5000, 7500 and 10000 mg/l
Details on test conditions:
TEST SYSTEM
- Test vessel: Erlenmeyer vessels (300 ml)
- Fill volume: 100 ml
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

Results and discussion

Effect concentrationsopen allclose all
Duration:
17 h
Dose descriptor:
EC10
Effect conc.:
6 532 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth inhibition
Duration:
17 h
Dose descriptor:
EC50
Effect conc.:
7 854 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth inhibition

Applicant's summary and conclusion