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EC number: 225-063-3 | CAS number: 4637-24-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Long-term toxicity to fish
Administrative data
- Endpoint:
- fish early-life stage toxicity
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Meets generally accepted scientific standards, well documented and acceptable for assessment
Data source
Reference
- Reference Type:
- publication
- Title:
- An artificial fertilization method with the Japanese medaka: Implications in early life stage bioassays and solvent toxicity
- Author:
- Gonzáles-Doncel, M. et al.
- Year:
- 2 008
- Bibliographic source:
- Ecotoxicology and Environmental Safety 69: 95-103
Materials and methods
Test guideline
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- Early lifestage bioassay. An in vitro fertilization method was used to study the effects on Medaka embryos reared either from 0.5 h or 6.5 h post-fertilization for 200 h.
- GLP compliance:
- not specified
Test material
- Reference substance name:
- Methanol
- EC Number:
- 200-659-6
- EC Name:
- Methanol
- Cas Number:
- 67-56-1
- IUPAC Name:
- methanol
- Details on test material:
- - Name of test material (as cited in study report): Methanol
- Analytical purity: >99%
Constituent 1
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
no data
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: nominal values of 0.30, 0.50, and 2.00% v/v were measured through time (from 0 - 168 h) in 20 mL vials containing 2 mL of the respective nominal concentrations
Test solutions
- Vehicle:
- no
- Details on test solutions:
- no details
Test organisms
- Test organisms (species):
- Oryzias latipes
- Details on test organisms:
- TEST ORGANISM
- Common name: Medaka
- Strain: orange-red strain
- Source: laboratory by first author
- Age at study initiation (mean and range, SD): 0.5 (embryo stage 2) and 6.5 h (embryo stage 10) post-fertilization
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 200 h
- Post exposure observation period:
- 72 h
Test conditions
- Hardness:
- no data
- Test temperature:
- 25 ± 1°C
- pH:
- no data
- Dissolved oxygen:
- no data
- Salinity:
- no data
- Nominal and measured concentrations:
- 474, 1027, 1975, 3950, 7900, 11850, 15800 mg/L (nominal) (re-calculated from % v/v data provided)
- Details on test conditions:
- TEST SYSTEM
- Test vessel: vials containing 2 mL test solution
- Renewal rate of test solution (frequency/flow rate): without renewal
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 5
- No. of vessels per control (replicates): 5
- No. of vessels per vehicle control (replicates): 5
- all experiments were run twice
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Rearing medium (RM)
OTHER TEST CONDITIONS
- Photoperiod: 16 h light, 8 h dark
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
daily: mortality (absence of heartbeat and blood stagnation), hatching failure (unable to hatch by day 21 after fertilization), any deviation from normal development - Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 200 h
- Dose descriptor:
- EC50
- Effect conc.:
- 14 536 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- number hatched
- Remarks:
- for stage 2
- Remarks on result:
- other: 95% CL: 12798-16590
- Duration:
- 200 h
- Dose descriptor:
- EC50
- Effect conc.:
- 9 164 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- other: % Larva swim bladder inflation for stage 2
- Remarks on result:
- other: 95% CL: 7900-10586
- Duration:
- 200 h
- Dose descriptor:
- EC50
- Effect conc.:
- 10 270 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- other: % Larva swim bladder inflation for stage 10
- Remarks on result:
- other: 95% CL: 9322-11376
- Duration:
- 200 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 11 850 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- other: % Embryo-cardiovascular for stage 2
- Duration:
- 200 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 39 505 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- number hatched
- Remarks:
- for stage 2
- Duration:
- 200 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 7 900 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- other: % Larva swim bladder inflation for stage 2
- Duration:
- 200 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 11 850 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- other: % Embryo-cardiovascular for stage 10
- Duration:
- 200 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 11 850 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- number hatched
- Remarks:
- for stage 10
- Duration:
- 200 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 7 900 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- other: % Larva swim bladder inflation for stage 10
- Duration:
- 200 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 15 800 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- other: % Embryo-cardiovascular for stage 2
- Duration:
- 200 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 7 900 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- number hatched
- Remarks:
- for stage 2
- Duration:
- 200 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 11 850 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- other: % Larva swim bladder inflation for stage 2
- Duration:
- 200 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 15 800 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- other: % Embryo-cardiovascular for stage 10
- Duration:
- 200 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 158 000 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- number hatched
- Remarks:
- for stage 10
- Duration:
- 200 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 11 850 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- other: % Larva swim bladder inflation for stage 10
- Duration:
- 200 h
- Dose descriptor:
- other: MATC
- Effect conc.:
- 13 667 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- other: % Embryo-cardiovascular for stage 2
- Duration:
- 200 h
- Dose descriptor:
- other: MATC
- Effect conc.:
- 9 638 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- other: % Larva swim bladder inflation for stage 2 and 10 each
- Duration:
- 200 h
- Dose descriptor:
- other: MATC
- Effect conc.:
- 5 609 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- number hatched
- Remarks:
- for stage 2
- Duration:
- 200 h
- Dose descriptor:
- other: MATC
- Effect conc.:
- 13 667 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- other: % Embryo-cardiovascular for stage 10
- Duration:
- 200 h
- Dose descriptor:
- other: MATC
- Effect conc.:
- 13 667 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- number hatched
- Remarks:
- for stage 10
- Details on results:
- Based on anatomical abnormalities no differences between both time windows (stage 2 and 10) were observed at concentrations of methanol ≤ 1975 mg/L. Only hatching success (EC50 14536 mg/L) points to the earlier window of exposure as significantly more sensitive.
- Reported statistics and error estimates:
- In an effort to normalize the distributions and to stabilize the variances, proportional data were transformed using the arcsine square root and continuous data were log-transformed. Differences from the controls and between treatments were identified with Dunnet’s procedure and Tukey–Kramer’s analysis (P < 0.05), respectively. When data were not normally distributed, the Wilcoxon’s sign-rank test was applied (P < 0.05). The designated known control vial used in each assay as temporal reference for normal development was excluded from statistical analysis. For the analytical studies, differences between sample concentrations were identified with one-way ANOVA and the Wilcoxon’s sign-rank test (P < 0.05). These tests were done using JMP statistical software (SAS, Cary, NC).
Any other information on results incl. tables
All effect concentrations given under results and discussions were re-calculated from % v/v data provided in publication.
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.