Ditolyl ether

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: scientific acceptable and well documented

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1984

Report date:

1984

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 403 (Acute Inhalation Toxicity)

Principles of method if other than guideline:

Ca. 200 l air per hour were conducted through ca. 100 ml of the test substance which was heated to 60°C. The air aerated in this way was applied to 5 male or 5 female rats. the animals were housed in a 10 l glass bottle and were exposed whole body to the test substance for 7 hours. Post-exposure period was 14 days.

GLP compliance:

yes

Test type:

other: inhalation hazard test

Test material

Test material information

Constituent 1

Reference substance name:

Ditolyl ether

EC Number:

248-948-6

EC Name:

Ditolyl ether

Cas Number:

28299-41-4

Molecular formula:

C14H14O

IUPAC Name:

Benzene, 1,1'-oxybis[methyl-

Details on test material:

content: 99.8%; density: 1.035 kg/liter (according LXS MSDS)

Test animals

Species:

rat

Strain:

Wistar

Sex:

male/female

Details on test animals or test system and environmental conditions:

male Wister rats, weight ca. 160 - 220 g, husbandry: standardised conditions, 5 animals per cage (makrolon type III)

Administration / exposure

Route of administration:

inhalation: vapour

Type of inhalation exposure:

whole body

Vehicle:

other: unchanged (no vehicle)

Details on inhalation exposure:

Animals were inspected several times on the same day of the application of the test substance. During the post observation period of 14 days animals were inspected twice daily and time of onset, duration, and severity of clinical signs recorded

Analytical verification of test atmosphere concentrations:

no

Duration of exposure:

7 h

Concentrations:

test substance was evaporated at 60°C

No. of animals per sex per dose:

5 male and 5 female rats

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: During the post observation period of 14 days animals were inspected twice daily and time of onset, duration, and severity of clinical signs recorded, animals were weighened before, after 1 week and at the end of the post-observation period
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight, pathology

Statistics:

not applicable - all animals survived

Results and discussion

Effect levels

Effect levels 1

Sex:

female

Dose descriptor:

discriminating conc.

Effect level:

> 521 mg/m³ air (nominal)

Exp. duration:

7 h

Effect levels 2

Sex:

male

Dose descriptor:

discriminating conc.

Effect level:

> 671 mg/m³ air (nominal)

Exp. duration:

7 h

Mortality:

all animals survived

Clinical signs:

other: no signs of toxicity were observed

Body weight:

male rats, 671 mg/m³; animal 1: start 193 g ,end 212 g; animal 2: start 186 g ,end 202 g; animal 3: start 203 g ,end 232 g; animal 4: start 200 g ,end 226 g; animal 1: start 188 g ,end 203 g
fwmale rats, 521 mg/m³; animal 1: start 196 g ,end 194 g; animal 2: start 200 g ,end 201 g; animal 3: start 199 g ,end 198 g; animal 4: start 194 g ,end 185 g; animal 1: start 203 g ,end 205 g

Gross pathology:

no findings concerning organ injuries caused by the test substance

Other findings:

no data

Any other information on results incl. tables

No mortality and no signs of toxicity, delayed effects were not observable

Applicant's summary and conclusion

Interpretation of results:

study cannot be used for classification

Executive summary:

Method: ca. 200 l air per hour were conducted through ca. 100 ml of the test substance which was heated to 60°C. the air aerated in this way was applied to 5 male or 5 female rats. the animals were housed in a 10 l glass bottle and were exposed hole body to the test substance for 7 hours. Post-exposure period was 14 days

Result: LC50 > 521 mg/m³ (rat, female); LC50 > 671 mg/m³ (rat, male), the whole body exposition for 7 hours of the saturated test atmosphere caused no mortality and no signs of toxicity were observable

Reference: Pauluhn (Bayer AG), 1984