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Toxicity to soil macroorganisms except arthropods

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Description of key information

The toxicity of sodium 3(2H-benzotriazol-2-yl)-5-sec-butyl-4-hydroxybenzenesulfonate towards Eisenia foetida was tested for a  exposure of 14 days according to OECD 207 guideline. The LC50 (7,14 days) of test item was > 1000 mg/kg.

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The toxicity of sodium 3(2H-benzotriazol-2-yl)-5-sec-butyl-4-hydroxybenzenesulfonate towards Eisenia foetida was tested for a exposure of 14 days

according to OECD 207 guideline. During the entire study, the pH of the articial soil was in a range of 5.5 to 5.8. The water capacity of the artificial soil was determined to be 50 % of the dry weight at the beginn of the study. The water content was ca. 35 - 36 % (dry weight) at start and in a range from 24 % to 36 % at the end of the study.

Nearly no worm died during of the entire study in the test vessels with test substance as well as in the water treated controls. Consequently it was not possible to calculate the LC50 of test item to Eisenia foetida, which is clearly higher than 1000 mg/kg artificial soil.

In all test concentrations, the body weights of the surviving worms decreased slightly due to the starvation of the animals within the test period. No behavioural abnormalities could be observed.

The LC50 (7,14 days) of sodium 3(2H-benzotriazol-2-yl)-5-sec-butyl-4-hydroxybenzenesulfonate was > 1000 mg/kg.

The results of this study did not reveal any toxic effect of test item to E. foetida at the limit concentration of 1000 mg/kg.