3-amino-p-toluamide

Toxicity to microorganisms

Administrative data

Endpoint:

toxicity to microorganisms

Type of information:

experimental study

Adequacy of study:

supporting study

Study period:

2014

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Toxicity test to microorganisms performed within OECD 301F (Ready Biodegradability Manometric Respirometry Test)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2014

Report date:

2014

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

3-amino-p-toluamide

EC Number:

243-039-0

EC Name:

3-amino-p-toluamide

Cas Number:

19406-86-1

Molecular formula:

C8H10N2O

IUPAC Name:

3-amino-p-toluamide

Test organisms

Test organisms (species):

other: activated sludge, non-adapted

Details on inoculum:

- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Activated sludge from the municipal sewage treatment plant, 31137 Hildesheim, Germany
- Preparation of inoculum for exposure: The activated sludge was washed twice with chlorine free tap water. After the second washing the settled sludge was resuspeded in min-eral salts medium and was maintained in an aerobic condition by aera-tion for 2 hours. Thereafter the sludge was homogenized with a blender. After sedimentation the supernatant was decanted and main-tained in an aerobic condition by aeration with CO2-free air for 6 days. 10 mL/L were used to initiate inoculation.
- Initial cell/biomass concentration: 4.2 x 10e9 CFU/L
- Water filtered: no

Study design

Water media type:

saltwater

Limit test:

yes

Total exposure duration:

28 d

Test conditions

pH:

7.75 (start) - 7.96 (end)
- pH adjusted: no

Nominal and measured concentrations:

Toxicity Control
28mg/L Test Item + 45 mg/L Reference Item (nominal)

Details on test conditions:

Toxicity test done within OECD301F:

TEST CONDITIONS
- Composition of medium: Mineral salts medium acc. to OECD 301F
- Additional substrate: No
- Test temperature: Nominal: 20 - 24, ± 1 °C; Actual: 21.4 – 21.7 °C
- pH:
pH-Values at Test Start and Test End

pH-Value
Start End
Inoculum Control 7.77 1) 7.73
2) 7.75
Functional Control 7.77 7.99
Test Item 7.74 1) 7.73
2) 7.74
Toxicity Control 7.75 7.96

- pH adjusted: no
- Dispersion treatment: Continuous stirring
- Continuous darkness: Dark, in an incubator


TEST SYSTEM
- Culturing apparatus: Incubator
- Number of culture flasks/concentration: 2
- Method used to create aerobic conditions: Closed bottles with a rubber sleeve with soda lime
- Measuring equipment: The bottles were closed with OxiTOP measuring heads.
The oxygen consumption was determined in the incubation vessels by the OxiTop® measuring system at 360 measuring points (every 112 minutes) during the 28 d incubation period.



SAMPLING
- Sampling frequency: 360 measuring points (every 112 minutes) during the 28 d incubation period.
- Sampling method: Pressure measurements directly from the test vessels



CONTROL AND BLANK SYSTEM
- Inoculum blank: Test medium without test and reference item
- Abiotic sterile control: No
- Toxicity control: Test item and reference item in test concentrations



STATISTICAL METHODS:
Calculation of the theoretical oxygen demand (ThOD) of the test item and functional control.
According to OECD-guideline 301, the index of the atoms of the item CcHhClclNnNanaOoPpSs was used for the calculation of the ThOD.
The oxygen depletion of the test and reference item was calculated out of the difference of the test and inoculum control replicates.
The biodegradation of the test and reference item was calculated out of the BOD in relation to the ThOD.

Reference substance (positive control):

yes

Remarks:

benzoic acid, sodium salt

Results and discussion

Any other information on results incl. tables

In the toxicity control the biodegradation achieved 53 % after 14 days. After 28 days the biodegradation came to 56 %.

Replicate	Biodegradation [%]
	Study Day [d ]
	7	14	21	28
Toxicity Control: 28mg/L Test Item + 45 mg/L Reference Item	48	53	54	56

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Remarks:

The percentage degradation of the toxicity control reached the pass level of 25 % within 2 days (validity criterion: 25 % after 14 days).

Conclusions:

In the toxicity control containing both test and reference item 53 % degradation occurred within 14 days. After 28 days the
biodegradation came to 56 %. The degradation of the reference item was not inhibited by the test item.