Registration Dossier

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
migrated information: read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP guidline study
Justification for data waiving:
other:

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2014
Report Date:
2014

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
yes (incl. certificate)

Test material

Reference
Name:
Unnamed
Type:
Constituent
Type:
Constituent
Details on test material:
Identification: 3-Amino-butan-1-ol
Chemical name: 1-Butanol, 3-amino-
CAS: 2867-59-6
Physical form: liquid
Appearance: clear, colouless
Water solubility: miscible
Purity: 93.6 corr. area %
Molecular weight: 89.137 g/mol
Density: 0.9440 ± 0.0001 (20°C)

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
Sampling at the start of the exposure (0h) was carried out immediately after preparation of the limit concentration (un-neutrolised and pH-neutrolised). At the end of the exposure (48h), samples were taken directly from the test vessels, which contained the daphinds.

Test solutions

Vehicle:
no
Details on test solutions:
A stock solution (100 mg/l of the test item was weighed out) was freshly prepared with dilution water in a measuring flask. The limit concentration of 100 mg/l was initially adjsuted to pH 7 ± 0.2 by the addition of 1 M HCl and tested under the same conditions as an un-neutralised trial with a limit concentration of 100 mg/l. The limit concentration (un-neutralised and pH-neutralised) was selected based on the results of a non GLP preliminary range finding test conducted at the test facility under static conditions.

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: Daphnia magna STRAUS
- Strain: Clone 5
- Source: Institut für Wasser-, Boden- und Lufthygiene (WaBoLu)
- Age at study initiation (mean and range, SD):
- Weight at study initiation (mean and range, SD):
- Length at study initiation (length definition, mean, range and SD):
- Valve height at study initiation, for shell deposition study (mean and range, SD):
- Peripheral shell growth removed prior to test initiation:
- Method of breeding: In glass vessels (2 -3 l capacity) with approximately 1.8 l culute medium at 20 ± 2°C, in an incubator, 16 h illumination; light intensity of max. 20µEm-2s-1
- Feeding during test: no

Study design

Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
48 h

Test conditions

Hardness:
178 mg CaCO3/l
Test temperature:
18 - 22°C, constant within ± 1°C
pH:
7.26 - 10.05
Dissolved oxygen:
8.24 - 8.85 mg/l
Details on test conditions:
20 daphnids, divided into 4 replicates, each with 5 daphnids, were used for the limit concentration (un-neutralised and pH-neutralised) and the control. Per replicate, 20 g of each test solution was weighed out into the test vessel. This corresponds to 20 ml per test vessel. The daphnids were inserted with a small amount of dilution water by a pipette.

Results and discussion

Effect concentrations
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
meas. (initial)
Conc. based on:
test mat.
Basis for effect:
mobility

Applicant's summary and conclusion