3-[(4-chloro-2-nitrophenyl)azo]-2-methylpyrazolo[5,1-b]quinazolin-9(1H)-one

Acute Toxicity: other routes

Administrative data

Endpoint:

acute toxicity: other routes

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Well documented study report.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1981

Report date:

1981

Materials and methods

Test guideline

Qualifier:

no guideline available

Principles of method if other than guideline:

acute toxicity upon intraperitoneal application

GLP compliance:

no

Limit test:

yes

Test material

Test material information

Constituent 1

Reference substance name:

3-[(4-chloro-2-nitrophenyl)azo]-2-methylpyrazolo[5,1-b]quinazolin-9(1H)-one

EC Number:

277-823-9

EC Name:

3-[(4-chloro-2-nitrophenyl)azo]-2-methylpyrazolo[5,1-b]quinazolin-9(1H)-one

Cas Number:

74336-59-7

Molecular formula:

C17H11ClN6O3

IUPAC Name:

3-[(4-chloro-2-nitrophenyl)diazenyl]-2-methylpyrazolo[5,1-b]quinazolin-9(1H)-one

Details on test material:

- Name of test material (as cited in study report): Pigmentorange Kan 78/1775
- Test substance number: 80/404
- Physical state: solid, orange
- Analytical purity: > 98 %
- Lot/batch No.: Batch 8

Test animals

Species:

mouse

Strain:

NMRI

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Willi Gassner, Sulzfeld, Germany
- Age at study initiation: about 4 weeks
- Mean weight at study initiation: 22 g (males), 20 g (females)
- Fasting period before study: 16 hours before administration, but water is available ad libitum.
- Housing: 5 per cage, Type IV Makrolon cages
- Diet: Herilan, mouse/rat/hamster maintenance diet; H. Eggersmann KG, Rinteln, Germany; ad libitum
- Water: Demineralized water each workday, tap water on holidays; ad libitum
- Acclimation period: at least 1 week

ENVIRONMENTAL CONDITIONS
- Temperature (°C): 20-26
- Humidity (%): 45-75
- Photoperiod (hrs dark / hrs light): 12/12

Administration / exposure

Route of administration:

intraperitoneal

Vehicle:

CMC (carboxymethyl cellulose)

Remarks:

0.5 % aqueous carboxymethyl cellulose

Details on exposure:

VEHICLE
- Concentration in vehicle: 20 % in 0.5% aqueous carboxymethyl cellulose
- Justification for choice of vehicle: Aqueous formulation corresponds to the physiological medium
- Form of administration: Suspension

MAXIMUM DOSE VOLUME APPLIED: 10 mL/kg bw

Doses:

2000 mg/kg bw

No. of animals per sex per dose:

5

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations: Recording of signs < 15, 15 and 30 minutes, 1 h, 2 h, 4 h and 5 h after administration of test substance and then once each workday. Check for moribund and dead animals twice each workday and once on weekends and public holidays.
- Frequency of weighing: Animals of comparable weights (± 10 g) in one cage. Group weighing before administration, 2nd weighing on days 2 - 4, 3rd weighing on day 7, 4th weighing on day 13 after administration.
- Necropsy of survivors performed: yes, withdrawal of feed 16 hours before sacrifice with CO2 then necropsy with gross-pathological
assessment. All animals that died are necropsied as early as possible.
- Other examinations performed: clinical signs, body weight

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

> 2 000 mg/kg bw

Based on:

test mat.

Remarks on result:

other: No mortality.

Mortality:

No mortality observed (0/10 animals).

Clinical signs:

Males and females: Dyspnea, apathy, staggering, stretching, ruffled fur and poor general state 5 hours after treatment up to 2 days

Body weight:

No abnormalities in body weight gain detected.
mean body weight:
- day 0: males: 22 g, females: 20 g;
- day 2-4: males: 23.6 g, females: 21 g;
- day 7: males: 27.0 g, females: 25.0 g;
- day 13: males: 28.4 g, females: 26.4 g.

Gross pathology:

Intra-abdominal incorporations of test substance in sacrificed animals detected.

Other findings:

No other findings.