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EC number: 800-906-3 | CAS number: 1402434-48-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
Description of key information
In the absence of a standard microbial inhibition test, the ready biodegradation test (Closed Bottle test) was used to assess the toxicity of the substance to microorganisms.
Key value for chemical safety assessment
- EC10 or NOEC for microorganisms:
- 2 mg/L
Additional information
The ready biodegradability of C12-14 alkylmorpholine was determined in the closed bottle test performed according to the OECD Guideline 301D, EU method C.4 and ISO 10707 (1994) in compliance with GLP with few minor deviation. The test was prolonged because the pass level was not reached at Day 28. The test substance was exposed to the inoculum, consisting of relatively low numbers of microorganisms from an activated sludge plant, under aerobic conditions for a period of at least 28 d. The concentrations of the test substance and the reference substance, sodium acetate in the BOD (biological oxygen demand) bottles were 2.0 and 6.7 mg/L, respectively. The test substance did not cause a degradation of sodium acetate as well as reduction in the endogenous respiration, nor inhibited the microorganisms degrading acetate. Therefore, no inhibition of the biodegradation due to the high initial concentration of the test substance is expected. The validity of the test is demonstrated by an endogenous respiration of 0.9 mg/L at Day 28. Furthermore, the differences of the replicate values at Day 28 were less than 20%. The biodegradation percentage of the reference substance, sodium acetate, at Day 14 was 80. Finally, the validity of the test is shown by oxygen concentrations >0.5 mg/L in all bottles during the test period. The test substance is biodegraded by 64% at day 28 in the test. The pass level of 60% was not reached within 14 d upon achieving 10% biodegradation. The reason for failing the 14-day window is the chemical structure, a surfactant consisting of a hydrophilic group linked to a hydrophobic moiety. Biodegradation of both moieties of surfactants requires the concerted action of at least two microorganisms as a single organism usually lacks the full complement of enzymatic capabilities. In ready biodegradability tests, the two moieties of this fatty amine derivative are therefore degraded sequentially. The time window criterion was developed on the assumption that a substance is degraded by one microorganism according to the “standard” growth curve. The degradation curve of the test substance is the sum of growth curves of the two moieties of the test substance i.e. morpholine and the alkyl chain. The biodegradability of the two moieties in the closed bottle test may be fully in line with the time window criterion when judged as separate substances. The time window should therefore be ignored as a pass fail criterion for the test substance.
Under the study conditions, the test substance is classified as readily biodegradable only based on the biodegradation percentage of 64% at Day 28.
The test substance was exposed to the inoculum, consisting of relatively low numbers of microorganisms from an activated sludge plant, under aerobic conditions for a period of at least 28 d. The concentrations of the test substance and the reference substance, sodium acetate in the BOD (biological oxygen demand) bottles were 2.0 and 6.7 mg/L, respectively.
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