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Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

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Description of key information

The 72 h ErC10 and ErC50 of the test substance to the freshwater algae,Pseudokirchneriella subscapitata , based on geometric measured mean were 6 and 25 µg/L, respectively. The NOEC was calculated as <5.7 µg/L and the LOEC as 5.7 µg/L.

Key value for chemical safety assessment

EC50 for freshwater algae:
25 µg/L

Additional information

A study was conducted to determine the acute toxicity of C12-C14 alkylmorpholine to the freshwater algae, Pseudokirchneriella subscapitata according to the OECD Guideline 201 in compliance with GLP. The test substance was exposed to an exponentially growing culture of P. subcapitata over an exposure period of 72 h. Nominal concentrations of 0, 31.3, 100, 320, 1020 and 3200 µg/L were tested. All test concentrations were tested in triplicate. In addition, six replicates of the control and a parallel replicate of every concentration without algae were included. Analytical results demonstrated that the test substance is adsorbing and also instable but still capable of causing significant toxic effect. Hence, geometric measured mean values were used as far as possible for calculating the endpoints. Where this was not possible half of the measured initial concentration was used. For all concentrations, a statistically significant effect to growth rate in comparison to the control was detected. Under the conditions of the study, the 72 h ErC10 and ErC50 of the test substance for P. subscapitata, based on geometric measured mean were 6 and 25 µg/L, respectively. The NOEC was calculated as <5.7 µg/L and the LOEC as 5.7 µg/L.