Methyl [(dimethoxyphosphinothioyl)thio]acetate

Eye irritation

Administrative data

Endpoint:

eye irritation: in vivo

Type of information:

experimental study

Adequacy of study:

key study

Study period:

From 29-09-1992 to 04-10-1992

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1992

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 405 (Acute Eye Irritation / Corrosion)

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Methyl [(dimethoxyphosphinothioyl)thio]acetate

EC Number:

212-058-6

EC Name:

Methyl [(dimethoxyphosphinothioyl)thio]acetate

Cas Number:

757-86-8

Molecular formula:

C5H11O4PS2

IUPAC Name:

methyl 2-{[dimethoxy(sulfanylidene)-λ⁵-phosphanyl]sulfanyl}acetate

Details on test material:

- Name of test material (as cited in study report): MPEM
- Substance type: Intermediate
- Physical state: Liquid
- Purity test date:
- Lot/batch No.: 20825

Test animals / tissue source

Species:

rabbit

Strain:

other: SPF albino of the stock Mol:Russian, females

Details on test animals or tissues and environmental conditions:

TEST ANIMALS
- Source: Møllegaard Breeding Centre Ltd, Ejby, DK-4623 Ll. Skensved, Denmark
- Age at study initiation: not known
- Weight at study initiation: 2.4 to 2.5 kg
- Fasting period before study: -
- Housing: single PPL cages, 45 cm x 55 cm, perforated floor.
- Diet : ad libitum "Altromin 2123" from Compagny Altromin GmbH D-4937 Lage, Lippe, Germany
- Water : ad libitum, acidified with hydrochloric acid to pH 2,5.
- Acclimation period: one week

ENVIRONMENTAL CONDITIONS
- Temperature (°C): 21, ± 3
- Humidity (%):55, ± 15
- Air changes (per hr): 10 times
- Photoperiod (hrs dark / hrs light): 12/12

Test system

Vehicle:

unchanged (no vehicle)

Controls:

other: only one eye on each rabbit treated, the other eye served as control

Amount / concentration applied:

TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 0,1 ml
- Concentration (if solution): pure

Duration of treatment / exposure:

24 hours

Observation period (in vivo):

over 72 hours, observations at 1 hour, 24 hours, 48 hours and 72 hours after exposure

Number of animals or in vitro replicates:

4

Details on study design:

REMOVAL OF TEST SUBSTANCE
- Washing (if done): yes, with 20 ml 0,9 % sodium chloride water
- Time after start of exposure: at 24 hours after exposure

SCORING SYSTEM: As in OECD guideline 405

TOOL USED TO ASSESS SCORE: oculoguttae fluoresceini, UV light to detect corneal damage

Results and discussion

In vivo

Results

Results 1

Irritation parameter:

cornea opacity score

Basis:

mean

Time point:

other: 24, 48 and 72 hours

Score:

0

Max. score:

1

Reversibility:

fully reversible within: 72 hours

Results 2

Irritation parameter:

iris score

Basis:

mean

Time point:

other: 24, 48 and 72 hours

Score:

0.1

Max. score:

1

Reversibility:

fully reversible within: 72 hours

Results 3

Irritation parameter:

chemosis score

Remarks:

oedema of conjunctiva (chemosis)

Basis:

mean

Time point:

other: 24, 74 and 72 hours

Score:

0.4

Max. score:

2

Reversibility:

fully reversible within: 72 hours

Results 4

Irritation parameter:

conjunctivae score

Remarks:

redness of

Basis:

mean

Time point:

other: 24, 48 and 72 hours

Score:

0.2

Max. score:

1

Reversibility:

fully reversible within: 72 hours

Irritant / corrosive response data:

At the 72 hours reading/observation, no reaction was observed in the eyes of any of the 4 rabbits used for this experiment

Applicant's summary and conclusion

Interpretation of results:

not irritating

Remarks:

Migrated information Criteria used for interpretation of results: EU

Conclusions:

Based on the result from this study, MPEM can not be classified as eye irritating